Protein Info for IAI46_06100 in Serratia liquefaciens MT49

Annotation: glutamine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 PF00749: tRNA-synt_1c" amino acids 28 to 337 (310 residues), 426.6 bits, see alignment E=7.2e-132 TIGR00440: glutamine--tRNA ligase" amino acids 28 to 551 (524 residues), 1017.1 bits, see alignment E=0 PF03950: tRNA-synt_1c_C" amino acids 340 to 439 (100 residues), 113.8 bits, see alignment E=5.3e-37 PF20974: tRNA-synt_1c_C2" amino acids 456 to 528 (73 residues), 92.3 bits, see alignment E=3.8e-30

Best Hits

Swiss-Prot: 96% identical to SYQ_SERP5: Glutamine--tRNA ligase (glnS) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01886, glutaminyl-tRNA synthetase [EC: 6.1.1.18] (inferred from 96% identity to spe:Spro_1229)

MetaCyc: 87% identical to glutamine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamine--tRNA ligase. [EC: 6.1.1.18]

Predicted SEED Role

"Glutaminyl-tRNA synthetase (EC 6.1.1.18)" in subsystem tRNA aminoacylation, Glu and Gln (EC 6.1.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (554 amino acids)

>IAI46_06100 glutamine--tRNA ligase (Serratia liquefaciens MT49)
MSEAEARPTNFIRQIVDEDLASGKHTSVHTRFPPEPNGYLHIGHAKSICLNFGIAKDYQG
QCNLRFDDTNPVKEDIEFVESIKHDVEWLGFEWSGNVRYSSDYFDQLHQYAVELINKGLA
YVDELSPEQIREYRGSLTSPGKDSPYRGRSVEENLALFEKMRNGEFAEGSACLRAKIDMA
SPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRL
YDWVLDNITIPCHPRQYEFSRLNLEYAIMSKRKLNQLVTEKIVEGWDDPRMPTVSGLRRR
GYTAASIREFCQRIGVTKQDNNVEMVALEACIREELNDNAPRAMAVLDPVKIVIENMTSG
VEMVTMPNHPNKPEMGSREVPFSREVYIDRADFREEANKQYKRLVLGKEVRLRNAYVIKA
ERVEKDAEGEITTIFCSYDPDTLSKDPADGRKVKGVIHWVSAEHALPAEIRVYDRLFNVP
NPAAAEDFLATINPESLVIKHGFVEPSLATAQPEKAYQFEREGYFCADNRYSSAEHLVFN
RTVGLRDTWAKIGA