Protein Info for IAI46_06075 in Serratia liquefaciens MT49

Annotation: HAD-IIA family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 3 to 214 (212 residues), 62.4 bits, E=1.6e-20
• TIGR01460: HAD hydrolase, family IIA
  • amino acids 6 to 223 (218 residues), 212.3 bits, E=9.4e-67
• PF13344: Hydrolase_6
  • amino acids 6 to 105 (100 residues), 88.7 bits, E=4.7e-29
• PF13419: HAD_2
  • amino acids 163 to 220 (58 residues), 35.6 bits, E=2.1e-12
• PF13242: Hydrolase_like
  • amino acids 174 to 246 (73 residues), 80.4 bits, E=1.5e-26

Best Hits

Swiss-Prot: 86% identical to NAGD_ECO57: Ribonucleotide monophosphatase NagD (nagD) from Escherichia coli O157:H7

KEGG orthology group: K02566, NagD protein (inferred from 99% identity to spe:Spro_1224)

MetaCyc: 86% identical to UMP phosphatase (Escherichia coli K-12 substr. MG1655)
5'-nucleotidase. [EC: 3.1.3.5]

Predicted SEED Role

"Phosphatase NagD predicted to act in N-acetylglucosamine utilization subsystem" in subsystem Chitin and N-acetylglucosamine utilization

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• NAD salvage (plants) (6/11 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.5

Use Curated BLAST to search for 3.1.3.5

Protein Sequence (250 amino acids)

>IAI46_06075 HAD-IIA family hydrolase (Serratia liquefaciens MT49)
MTIKNVICDIDGVLLHDNTPVPGADLFLARIQEQGMPLVVLTNYPSQTAQDLANRFSAAG
LEVPESAFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKAGFTITDINPDFVIVGETR
SYNWDMMHKAAYFVNNGARFIATNPDTHGHGFVPACGALCAPIEKITGRKPFYVGKPSPW
IIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETVLVLSGVSTLNDVETMPFRPSYI
FPSVADINIF