Protein Info for IAI46_05975 in Serratia liquefaciens MT49

Annotation: pyrimidine-specific ribonucleoside hydrolase RihA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 5 to 300 (296 residues), 349.7 bits, E=9.1e-109

Best Hits

Swiss-Prot: 63% identical to RIHA_PHOPR: Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) from Photobacterium profundum (strain SS9)

KEGG orthology group: K01250, pyrimidine-specific ribonucleoside hydrolase [EC: 3.2.-.-] (inferred from 96% identity to spe:Spro_1210)

MetaCyc: 61% identical to pyrimidine-specific ribonucleoside hydrolase RihA (Escherichia coli K-12 substr. MG1655)
Uridine nucleosidase. [EC: 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.3]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Glycosaminoglycan degradation
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.-.- or 3.2.2.1 or 3.2.2.3

Protein Sequence (310 amino acids)

>IAI46_05975 pyrimidine-specific ribonucleoside hydrolase RihA (Serratia liquefaciens MT49)
MPRSIIIDCDPGLDDAIALAMALSSPELDVKAITTSAGNQTPEKTLHNALGLLTLMKRED
IPVAAGAAAPLMRDLVIADYVHGKTGMGNTHLPTPAIQPVAQTAVELIASLLRSSPQPIT
LVVTGPMTNIALLLAQHPELKGNIERIVFMGGGMNAGNTTPVAEFNIYVDPEAAEMVLKS
GVPLTMAGLNVTHQALVLPQDIERIRQINNPVAQAVAEMLDFYLPLYLSHPRGLPGAAMH
DPCTIAWLLAPQLFGSIERWVGVETKGEYTLGMTVVDIFGQNGKPANVEVLTDIDREGFI
DLLAERVARY