Protein Info for IAI46_05930 in Serratia liquefaciens MT49

Annotation: peptidoglycan DD-transpeptidase MrdA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 21 to 42 (22 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 20 to 610 (591 residues), 785.9 bits, see alignment E=1.3e-240 PF03717: PBP_dimer" amino acids 65 to 240 (176 residues), 165.6 bits, see alignment E=1.7e-52 PF00905: Transpeptidase" amino acids 273 to 606 (334 residues), 255.1 bits, see alignment E=8.6e-80

Best Hits

Swiss-Prot: 82% identical to MRDA_ECOL6: Peptidoglycan D,D-transpeptidase MrdA (mrdA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 97% identity to spe:Spro_1201)

MetaCyc: 82% identical to peptidoglycan DD-transpeptidase MrdA (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Penicillin-binding protein 2 (PBP-2)" in subsystem Peptidoglycan Biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4

Use Curated BLAST to search for 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (631 amino acids)

>IAI46_05930 peptidoglycan DD-transpeptidase MrdA (Serratia liquefaciens MT49)
MKIERNPFRDYTAESALFVRRALVAFLVILVLTGTLIANLYNLQIVRFEDYRTRSNENRI
KLVPIAPSRGIIYDRNGTPLALNRTIYQLELMPEKVDDLKATLQALRSVVDLTDDDITNF
EKERKRSRRFVSIPVKTALSEVQVARFAVNQFRFPGVEVKGYQRRYYPYGSALTHVIGYV
SKINDRDVERLDKDGKLANYAATHDIGKLGIERYYEETLHGKTGYEEVEVNNRGRVIRQL
HEQPPSAGKDVYLTLDLNLQRYIEQLLVGSRAAVVVSDPRTGGILAMVSNPSYDPNLFVD
GISSKDYQGLLNDPNRPLINRATQGLYPPASTVKPYISVSALSAGVITKNTIVFDPGWWQ
LPGSEKRFRDWKKWGHGRLNVTKALEESADTFFYQVAYDMGIDRLSTWLTKFGYGEYTGI
DLSEERSGLMPTRDWKFKRYKKPWYQGDTIPVGIGQGYWTATPIQMAKALTTLINDGNVK
TPHLLQSTRINGVLVPFQQEESTQIGDIHSGFWEIAKDGMYGVANRPNGTARKYFAGTPY
KAAAKSGTAQVFGYETYNAHKLAEHLRDHKLMTAFAPFDNPTVSVAIILENGGAGPAVGT
ITRQILDHILLGDNNTQLPDAAPLPPGVEGD