Protein Info for IAI46_05190 in Serratia liquefaciens MT49

Annotation: multidrug efflux RND transporter periplasmic adaptor subunit SdeX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00529: CusB_dom_1" amino acids 40 to 363 (324 residues), 62 bits, see alignment E=8.2e-21 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 42 to 378 (337 residues), 278.3 bits, see alignment E=3.6e-87 PF16576: HlyD_D23" amino acids 66 to 295 (230 residues), 47.1 bits, see alignment E=3.7e-16 PF13533: Biotin_lipoyl_2" amino acids 69 to 115 (47 residues), 24.5 bits, see alignment 3.7e-09 PF13437: HlyD_3" amino acids 177 to 292 (116 residues), 28.8 bits, see alignment E=3.4e-10

Best Hits

Swiss-Prot: 78% identical to ACRA_ECO57: Multidrug efflux pump subunit AcrA (acrA) from Escherichia coli O157:H7

KEGG orthology group: K03585, membrane fusion protein (inferred from 97% identity to srs:SerAS12_1055)

MetaCyc: 78% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Membrane fusion protein of RND family multidrug efflux pump" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (395 amino acids)

>IAI46_05190 multidrug efflux RND transporter periplasmic adaptor subunit SdeX (Serratia liquefaciens MT49)
MNKNRGLTPLAAVLMLSGSLVLTGCNDKETQQQGAQAPAPEVGVVTLKAEPLNITTDLPG
RTAAYRIAEVRPQVSGIILKRNFVEGSDIKAGTSLYQIDPATYQAAYDSAKGDLAKAQAS
ASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQAEAAVVAAKAAVETARINLAYTKVTS
PISGRIGKSAVTEGALVTNGQATALSTVQQLDPMYVDVTQSSNDFLRLKQELASGALKQE
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRAVFPNPNDTLLPGMFVRARLD
EGVRSDALLVPQQGVTRNPRGDATALVVGADDKVELRTLKADQAIGDKWLVTDGLKAGDR
VIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS