Protein Info for IAI46_04705 in Serratia liquefaciens MT49

Annotation: pyrimidine/purine nucleoside phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06865: Ppnp
  • amino acids 3 to 93 (91 residues), 109.2 bits, E=5.5e-36

Best Hits

Swiss-Prot: 98% identical to PPNP_SERP5: Pyrimidine/purine nucleoside phosphorylase (ppnP) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 97% identity to srr:SerAS9_0948)

MetaCyc: 73% identical to nucleoside phosphorylase PpnP (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.4]; Uridine phosphorylase. [EC: 2.4.2.2, 2.4.2.4, 2.4.2.3]; Purine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.4, 2.4.2.3, 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.2, 2.4.2.4, 2.4.2.3, 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.2, 2.4.2.4, 2.4.2.3, 2.4.2.1, 2.4.2.15]; 2.4.2.1 [EC: 2.4.2.2, 2.4.2.4, 2.4.2.3, 2.4.2.1, 2.4.2.15]

Predicted SEED Role

"Cytoplasmic protein YaiE" in subsystem CBSS-562.2.peg.5158 SK3 including

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of purine deoxyribonucleosides degradation (7/7 steps found)
• purine ribonucleosides degradation (6/6 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• adenine and adenosine salvage III (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• purine deoxyribonucleosides degradation I (4/4 steps found)
• ureide biosynthesis (6/7 steps found)
• adenine and adenosine salvage V (3/3 steps found)
• purine deoxyribonucleosides degradation II (3/3 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• superpathway of guanine and guanosine salvage (3/3 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• superpathway of pyrimidine ribonucleosides degradation (3/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
• arsenic detoxification (mammals) (8/17 steps found)
• salinosporamide A biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1, 2.4.2.2, 2.4.2.3, 2.4.2.4

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.15 or 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Protein Sequence (96 amino acids)

>IAI46_04705 pyrimidine/purine nucleoside phosphorylase (Serratia liquefaciens MT49)
MLKVNEYFAGKVKSIGFDSSSIGLTSVGVMEEGEYTFTTAQPEEMTVITGALKVLLPGSP
DWQVFMPGEKFFVPGHSEFNLQVADATAYLCRYLSK