Protein Info for IAI46_04435 in Serratia liquefaciens MT49

Annotation: glutamate-5-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 4 to 283 (280 residues), 52.1 bits, E=2.1e-18
• TIGR00407: glutamate-5-semialdehyde dehydrogenase
  • amino acids 9 to 406 (398 residues), 620.3 bits, E=6.5e-191

Best Hits

Swiss-Prot: 98% identical to PROA_SERP5: Gamma-glutamyl phosphate reductase (proA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00147, glutamate-5-semialdehyde dehydrogenase [EC: 1.2.1.41] (inferred from 98% identity to spe:Spro_0968)

MetaCyc: 76% identical to glutamate-5-semialdehyde dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate-5-semialdehyde dehydrogenase. [EC: 1.2.1.41]

Predicted SEED Role

"Gamma-glutamyl phosphate reductase (EC 1.2.1.41)" in subsystem Proline Synthesis (EC 1.2.1.41)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-ornithine biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.41

Protein Sequence (417 amino acids)

>IAI46_04435 glutamate-5-semialdehyde dehydrogenase (Serratia liquefaciens MT49)
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADRLEAESEVILQANEQDMTQARASGMSE
ALLDRLLLTPARLAAIANDVRQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYE
ARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQQALEQCGLPAAAVQAIDSPD
RALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
VIENAKIQRPSACNSLETLLVNRSIAAEFLPALSARMAAVGVTLHAAENAMPLLQDGPAT
VVAVAAEDYDDEWLSLDLNVALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVD
SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLDALTTYKWIGYGDDLVRS