Protein Info for IAI46_04430 in Serratia liquefaciens MT49

Annotation: glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 5 to 365 (361 residues), 506.7 bits, E=1.9e-156
• PF00696: AA_kinase
  • amino acids 6 to 234 (229 residues), 158.9 bits, E=1.9e-50
• PF01472: PUA
  • amino acids 276 to 350 (75 residues), 77.3 bits, E=7.1e-26

Best Hits

Swiss-Prot: 100% identical to PROB_SERP5: Glutamate 5-kinase (proB) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 99% identity to srr:SerAS9_0892)

MetaCyc: 89% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-N^δ-acetylornithine biosynthesis (5/7 steps found)
• L-ornithine biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (367 amino acids)

>IAI46_04430 glutamate 5-kinase (Serratia liquefaciens MT49)
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHL
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDT
MTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLYTADPR
NNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGI
REVKGDFSRGEVIRIRNLAGRDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVH
RDDMIVS