Protein Info for IAI46_04360 in Serratia liquefaciens MT49

Annotation: Na(+)-translocating NADH-quinone reductase subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 PF05896: NQRA" amino acids 1 to 259 (259 residues), 379.3 bits, see alignment E=1.5e-117 TIGR01936: NADH:ubiquinone oxidoreductase, Na(+)-translocating, A subunit" amino acids 2 to 449 (448 residues), 605.1 bits, see alignment E=4.1e-186 PF13375: RnfC_N" amino acids 18 to 84 (67 residues), 27 bits, see alignment E=5.6e-10 PF11973: NQRA_SLBB" amino acids 264 to 312 (49 residues), 78.6 bits, see alignment 6.1e-26

Best Hits

Swiss-Prot: 95% identical to NQRA_SERP5: Na(+)-translocating NADH-quinone reductase subunit A (nqrA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00346, Na+-transporting NADH:ubiquinone oxidoreductase subunit A [EC: 1.6.5.-] (inferred from 95% identity to spe:Spro_0953)

MetaCyc: 63% identical to Na(+)-translocating NADH-quinone reductase subunit A (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit A (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (449 amino acids)

>IAI46_04360 Na(+)-translocating NADH-quinone reductase subunit A (Serratia liquefaciens MT49)
MIKIRKGLDLPIAGTPVQAIHDGPIIQHVALLGEEYVGMRPSMLVQEGDSVKKGQALFED
KKTPGVFFTAPASGRIVAINRGERRVLQSVVIAIENGGDEQLEFAHYPMAELAMLPREQV
ESELIASGLWTALRTRPFSKTPVPGTTPRAIFVTAMDSQPLAADPLVIITEQLAAFNAGL
SVLARLTDGKVHVCHASGANLGKQPDAQVTYNEFAGPHPAGLVGTHIHFLEPVSLKKTVW
HIGYQDVIAIGTLFTTGKLDTDRVVALAGPQVELPQLLRTRLGASLDELTAGRLKAGENR
VISGSVLSGTHVAGPNAYLGRFHSQVSVLEEGRDKQLFGWIAPSPDKFSITRTTLGHFLK
NKLFAFSTTTHGGERAMVPIGNYERVMPLDILPTLLLRDLLAGDSDSAQALGCLELDEED
LALCTFVCPGKYEYAPVLRDVLTKIEQEG