Protein Info for IAI46_04135 in Serratia liquefaciens MT49

Annotation: hydroxyacylglutathione hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 251 TIGR03413: hydroxyacylglutathione hydrolase" amino acids 4 to 251 (248 residues), 342.4 bits, see alignment E=6.8e-107 PF00753: Lactamase_B" amino acids 13 to 165 (153 residues), 74.3 bits, see alignment E=2.7e-24 PF16123: HAGH_C" amino acids 166 to 251 (86 residues), 83.1 bits, see alignment E=3e-27

Best Hits

Swiss-Prot: 92% identical to GLO2_SERP5: Hydroxyacylglutathione hydrolase (gloB) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 91% identity to srr:SerAS9_0833)

MetaCyc: 62% identical to hydroxyacylglutathione hydrolase GloB (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]

Predicted SEED Role

"Hydroxyacylglutathione hydrolase (EC 3.1.2.6)" in subsystem Glutathione: Non-redox reactions or Methylglyoxal Metabolism (EC 3.1.2.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (251 amino acids)

>IAI46_04135 hydroxyacylglutathione hydrolase (Serratia liquefaciens MT49)
MNLISIPAFQDNYIWLLDNQQGHGIIVDPGEAQPVLDALERLNLTPDAILLTHHHHDHVG
GVAQIVARYPGLKVYGPQETADKGANHIVHDGETLEIDGRKFAVIAVPGHTLGHIAFYSA
PYLFCGDTIFSAGCGRLFEGTAEQMYDSFQQLAQLPDNTLICCAHEYTLSNLKFARAILP
EDRGIETYQQQVEALRAKGLPSVPTQLQLERKINLFLRCHDTDLQRKLGFHSPPQHLHSV
FSELRLRKDNF