Protein Info for IAI46_04130 in Serratia liquefaciens MT49

Annotation: murein transglycosylase D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01464: SLT" amino acids 112 to 223 (112 residues), 102.7 bits, see alignment E=1.5e-33 PF01476: LysM" amino acids 354 to 396 (43 residues), 48.7 bits, see alignment 8.9e-17 amino acids 408 to 451 (44 residues), 42.7 bits, see alignment 6.5e-15

Best Hits

Swiss-Prot: 63% identical to MLTD_ECOLI: Membrane-bound lytic murein transglycosylase D (mltD) from Escherichia coli (strain K12)

KEGG orthology group: K08307, membrane-bound lytic murein transglycosylase D [EC: 3.2.1.-] (inferred from 98% identity to spe:Spro_0908)

MetaCyc: 63% identical to membrane-bound lytic murein transglycosylase D (Escherichia coli K-12 substr. MG1655)
4.2.2.f [EC: 4.2.2.f]; 4.2.2.f [EC: 4.2.2.f]

Predicted SEED Role

"Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-)" (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 4.2.2.f

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>IAI46_04130 murein transglycosylase D (Serratia liquefaciens MT49)
MKAKAIFLASVLLVGCQSSRQDAPAPEQHAQSLSSAGQESEAGEYTANGRASSARWLDNN
SGAAQQNLWNFISDELKMEVPENSRIRDQKRKYLKSKSYLHDVTLRAEPYMYWIVGQIKK
RNMPMELVLLPIVESAFDPHATSSANAAGLWQIVPQTGRNYGLKNNQWYDGRRDVVASTT
AALDMMQYLNRMFNGDWLLTVAAYNSGEGRVMQAVKANKRQGKPTNFWALSLPRETSIYV
PKMLALSDIIKNSKKYGINLPKTDNTRALARIDVGQQIQLTQAAEMAGLSITKMKAYNPG
YKKGVTAPNGPHYIMVPKGHADQLKDSLADGQIAVTQPTTQLAKNSGLTGGSSYKVRSGD
TVSGIAKRLNIKSSDLQSWNNLRAKSTLKVGQTLQVASNTGSNSSITYQVRKGDSLASIA
RRHGVDINDVMRWNSTLAKANNLQPGLKLTLFVSDKSSPDT