Protein Info for IAI46_03960 in Serratia liquefaciens MT49

Annotation: bifunctional chorismate mutase/prephenate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 TIGR01799: chorismate mutase" amino acids 5 to 87 (83 residues), 148.1 bits, see alignment E=3.1e-48 PF01817: CM_2" amino acids 9 to 84 (76 residues), 85 bits, see alignment E=6e-28 PF02153: PDH_N" amino acids 138 to 218 (81 residues), 48.2 bits, see alignment E=1.3e-16 PF20463: PDH_C" amino acids 242 to 344 (103 residues), 48.1 bits, see alignment E=1.8e-16

Best Hits

Swiss-Prot: 86% identical to TYRA_ENTAG: T-protein (tyrA) from Enterobacter agglomerans

KEGG orthology group: K14187, chorismate mutase / prephenate dehydrogenase [EC: 1.3.1.12 5.4.99.5] (inferred from 99% identity to spe:Spro_0880)

MetaCyc: 86% identical to fused chorismate mutase/prephenate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Chorismate mutase. [EC: 5.4.99.5]; Prephenate dehydrogenase. [EC: 5.4.99.5, 1.3.1.12]

Predicted SEED Role

"Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 5.4.99.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.5

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 5.4.99.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (373 amino acids)

>IAI46_03960 bifunctional chorismate mutase/prephenate dehydrogenase (Serratia liquefaciens MT49)
MVAELTALRDQIDEVDKALLELLAKRLQLVAEVGEVKSHHGLPVYVPEREAAMLASRRQE
AENLGVPPDLIEDVLRRVMRESYVSENDKGFKTLHPQLRPIVIIGGNGQMGRLFNRLLTL
SGYQVKVLDQEDWNQADTLLADAGMVIVSVPIHVTEQVIERLPPLPADCILVDLASVKNR
PLNAMLAAHHGPVVGLHPMFGPDVGSVAKQVVVYCDGREPQAYQWLLEQLQVWGARLHRI
SAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQLLALSSPIYRLELAMVGRLFAQDP
QLYADIIMSSEENIALIKRYYQRFGEAIKLLEQGDKQAFIQSFQKVEHWFGDYAQRFLVE
SRSLLRQANDSRQ