Protein Info for IAI46_03850 in Serratia liquefaciens MT49
Annotation: alanine--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to SYA_CITK8: Alanine--tRNA ligase (alaS) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)
KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 85% identity to esc:Entcl_1030)MetaCyc: 84% identical to alanine--tRNA ligase/DNA-binding transcriptional repressor (Escherichia coli K-12 substr. MG1655)
Alanine--tRNA ligase. [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; 6.1.1.7 [EC: 6.1.1.7]; RXN-19726 [EC: 6.1.1.7, 3.1.1.96]; 3.1.1.- [EC: 6.1.1.7, 3.1.1.96]
Predicted SEED Role
"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)
MetaCyc Pathways
- superpathway of chorismate metabolism (54/59 steps found)
- tRNA charging (21/21 steps found)
- enterobactin biosynthesis (7/11 steps found)
- bacillibactin biosynthesis (6/12 steps found)
- shinorine biosynthesis (3/8 steps found)
- vanchrobactin biosynthesis (3/8 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (2/25 steps found)
- phosphinothricin tripeptide biosynthesis (2/25 steps found)
- corallopyronin A biosynthesis (2/30 steps found)
- colibactin biosynthesis (7/38 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 3.1.1.96 or 6.1.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (875 amino acids)
>IAI46_03850 alanine--tRNA ligase (Serratia liquefaciens MT49) MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRAYS RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAISFAWELLTGENW FNLPKERLWVTVYETDDEAFDIWQKQIGVPAERIIRIGDNKGAAFASDNFWQMGDTGPCG PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQSDGTMLPLPKPSVDTGMG LERIAAVLQHVNSNYEIDLFSTLIAAVAKVTGAVDLNNKSLRVIADHIRSCAFLVSDGVT PSNEGRGYVLRRIIRRAVRHGNMLGAKDSFFYKLVAPLIEVMGPAADELKRQQSLVEQVL KTEEDQFARTLERGLTLLDEELANLQGDTLDGETAFRLYDTYGFPVDLTADVCRERGLKV DEAGFELAMEAQRRRARESSGFGADYNSMIRVDGASQFCGYDHEQQQSTVIALFRDGQSV NEIHAGEEAVVVLDETPFYGESGGQVGDKGVLKAAGADFEVSDTQKYGQAIGHQGKLSQG SLKVNDRVDAQIDTVRRNRIRLNHSATHLLHAALRQTLGEHVAQKGSLVNDKYLRFDFSH FEAMKPEQIRIVEDLVNQQIRRNLPVQTEVMALDDAKEKGAMALFGEKYDDNVRVLTMGD FSTELCGGTHASRTGDIGLFRILSESGTAAGIRRIEAVTGEGAIATLHQQNDLLQDVAHL VKGDSNNLTDKVRAVLDRTRALEKELQQLKDQQAAQESASLSSKAKMVNGVPLLVSKLDN VEPKMLRTMVDDLKNQLGSAIIVLATTADDKVSLIAGVTKDLTDRVKAGELIGNVAQQVG GKGGGRPDMAQAGGTDVSALPAALDSVEAWVASKL