Protein Info for IAI46_03710 in Serratia liquefaciens MT49

Annotation: uroporphyrinogen-III C-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 207 (204 residues), 269.5 bits, see alignment E=1.8e-84 PF13241: NAD_binding_7" amino acids 6 to 115 (110 residues), 111.4 bits, see alignment E=6.1e-36 PF14824: Sirohm_synth_M" amino acids 119 to 145 (27 residues), 31.1 bits, see alignment (E = 2.7e-11) PF10414: CysG_dimeriser" amino acids 150 to 207 (58 residues), 71.2 bits, see alignment 9.3e-24 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 219 to 452 (234 residues), 301.8 bits, see alignment E=3.9e-94 PF00590: TP_methylase" amino acids 221 to 430 (210 residues), 181 bits, see alignment E=6e-57

Best Hits

Swiss-Prot: 97% identical to CYSG1_SERP5: Siroheme synthase 1 (cysG1) from Serratia proteamaculans (strain 568)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 97% identity to spe:Spro_0817)

MetaCyc: 57% identical to uroporphyrinogen-III C-methyltransferase subunit (Salmonella enterica enterica serovar Typhimurium)
Sirohydrochlorin ferrochelatase. [EC: 4.99.1.4]; Uroporphyrinogen-III C-methyltransferase. [EC: 4.99.1.4, 2.1.1.107]; 2.1.1.107 [EC: 4.99.1.4, 2.1.1.107]; Precorrin-2 dehydrogenase. [EC: 4.99.1.4, 2.1.1.107, 1.3.1.76]

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>IAI46_03710 uroporphyrinogen-III C-methyltransferase (Serratia liquefaciens MT49)
MDYLPIFADLKQRPVLVVGGGEVAARKVDLLLRAGAEIRIVAQSLSPILEELHQQGLIRW
QGQAFAAQQLDEVFLVIAATDDSALNAEVFAEADKRRVLANVVDDQPRCSFIFPSIIDRS
PLVVAVSSSGQAPVLARMLREKLEALLPASLGQMAEVAGRWRGQVKQRLNAIGERRRFWE
KTFGGRFATLVANGQSAQAERQLEQDLQQFAQGGEGTQGEIALVGAGPGDVGLLTLRGLQ
VMQQADVVLYDHLVSDEILDLVRRDAERICVGKRAGAHSVIQEETNRLLVELAQQGKRVV
RLKGGDPFIFGRGGEELQVAAAAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFIT
GHCRPDGDGLDWADLARARQTLAIYMGTMKAADISQRLIAHGRAATTPVAVISRGTRADQ
LVQTGTLQQLEKLAQQAPLPALLVIGEVVELHHQIAWFGHQPQTEGASRPAVVNLA