Protein Info for IAI46_03450 in Serratia liquefaciens MT49
Annotation: D-alanine--D-alanine ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to DDL_YERPB: D-alanine--D-alanine ligase (ddl) from Yersinia pseudotuberculosis serotype IB (strain PB1/+)
KEGG orthology group: K01921, D-alanine-D-alanine ligase [EC: 6.3.2.4] (inferred from 98% identity to srs:SerAS12_0697)MetaCyc: 72% identical to D-alanine--D-alanine ligase B (Escherichia coli K-12 substr. MG1655)
D-alanine--D-alanine ligase. [EC: 6.3.2.4]
Predicted SEED Role
"D-alanine--D-alanine ligase (EC 6.3.2.4)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.4)
MetaCyc Pathways
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
- UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
- peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.2.4
Use Curated BLAST to search for 6.3.2.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (306 amino acids)
>IAI46_03450 D-alanine--D-alanine ligase (Serratia liquefaciens MT49) MADKVAVLLGGTSAEREVSLQSGAAVLAGLREAGIDAHGIDIRDFPVTQLKEHGFTKVFI ALHGRGGEDGTLQGMLEFLELPYTGSGVMASALTMDKWRTKMVWQSMGLPVAPYVALNRQ QYAGAEKEALIARIEALGLPLIVKPSREGSSVGMSKVTERGALEAALEEGFRHDDDVLVE KWLSGPEYTVAMLGDRVLPSIRIQPAGVFYDYQAKYISDDTQYFCPSGLGAEQEAEMASL ALRAYRGLDCSGWGRVDVMQDSDGSFYLLEVNTSPGMTSHSLVPMAARQSGLSFSQLVAR ILELAD