Protein Info for IAI46_03430 in Serratia liquefaciens MT49

Annotation: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 signal peptide" amino acids 1 to 12 (12 residues), see Phobius details transmembrane" amino acids 13 to 27 (15 residues), see Phobius details PF21799: MurD-like_N" amino acids 7 to 94 (88 residues), 124.3 bits, see alignment E=3e-40 TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase" amino acids 8 to 435 (428 residues), 415.2 bits, see alignment E=1.9e-128 PF08245: Mur_ligase_M" amino acids 110 to 280 (171 residues), 89.4 bits, see alignment E=4.7e-29 PF02875: Mur_ligase_C" amino acids 301 to 370 (70 residues), 40.3 bits, see alignment E=4.7e-14

Best Hits

Swiss-Prot: 85% identical to MURD_SHIBS: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Shigella boydii serotype 4 (strain Sb227)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 97% identity to spe:Spro_0759)

MetaCyc: 85% identical to UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase. [EC: 6.3.2.9]

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>IAI46_03430 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (Serratia liquefaciens MT49)
MADYQGKKVVIIGLGLTGLSCVDFFMARGVTPRVMDTRISPPGLDKLSESVERHLGDLNQ
DWLLAADLIVASPGMALATPALSAAAEAGVEIVGDIELFCREAQAPIVAITGSNGKSTVT
TLVGEMAKAAGWAVGVGGNIGLPALSLLRQECQLYVLELSSFQLETTYSLHAAAATILNV
TEDHMDRYPFGLQQYRAAKLKVYENASLCVVNADDALTMPIRGADQRCISFGADVGDYHL
NRQLGETWLRVRGEKILNTREMKLTGRHNYTNALAALALADAVNIPRASSLKALTTYTGL
AHRFQLAWEHNGVRWINDSKATNVGSTEAALNGLQVDGTLHLLLGGDGKSADFSPLARYL
QGDNVRLYCFGRDGEQLAQLRPEVATLTETMEQAMQTIAGRVQPGDMVLLSPACASLDQF
RNFEVRGDEFTRLAQELGG