Protein Info for IAI46_03355 in Serratia liquefaciens MT49

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 4 to 273 (270 residues), 363.4 bits, E=7.8e-113
• TIGR00973: 2-isopropylmalate synthase
  • amino acids 4 to 497 (494 residues), 845.4 bits, E=6e-259
• PF08502: LeuA_dimer
  • amino acids 369 to 499 (131 residues), 137.6 bits, E=2.4e-44

Best Hits

Swiss-Prot: 99% identical to LEU1_SERP5: 2-isopropylmalate synthase (leuA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 99% identity to spe:Spro_0745)

MetaCyc: 84% identical to 2-isopropylmalate synthase (Escherichia coli K-12 substr. MG1655)
2-isopropylmalate synthase. [EC: 2.3.3.13]

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pyruvate metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Protein Sequence (524 amino acids)

>IAI46_03355 2-isopropylmalate synthase (Serratia liquefaciens MT49)
MSQQVIIFDTTLRDGEQALQASLSVKEKIQIAMALERMGVDVMEVGFPVSSPGDFESVQT
IARQIKNSRVCGLARCVDKDIDVAAEALRVAEAFRIHVFLATSTLHIESKLKRSFDEVLE
MAIRSVKRARNYTDDVEFSCEDAGRTPIDNLCRVVEAAINAGATTINIPDTVGYTTPNQF
GGIITTLYDRVPNIDKAIISVHCHDDLGMAVGNSIAAVQAGARQVEGTLNGIGERAGNTS
LEEVIMAIKVRQDIMNVHTNINHQEIFRTSQIVSQLCNMPIPANKAIVGSNAFAHSSGIH
QDGVLKNRENYEIMSPQTIGLKDVQLNLTSRSGRAAVKHRMEEMGYKEQDYNLDNLYAAF
LKLADKKGQVFDYDLEALAFINKQQEEPEHFSLDYFSVQSGSSIMATASVKLVCGEEEKA
EAATGNGPVDAVYQAINRITGYPIELVKYQLTAKGQGRDALGQVDIVVSYNGRRFHGVGL
ATDIVESSAKAMVHVLNNIWRSQQVEKEKQRLQQNKHQNNQETV