Protein Info for IAI46_03120 in Serratia liquefaciens MT49

Annotation: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00216: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • amino acids 2 to 285 (284 residues), 325.6 bits, E=1.2e-101
• PF02401: LYTB
  • amino acids 3 to 284 (282 residues), 330.8 bits, E=3.1e-103

Best Hits

Swiss-Prot: 98% identical to ISPH_SERP5: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) from Serratia proteamaculans (strain 568)

KEGG orthology group: K03527, 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [EC: 1.17.1.2] (inferred from 96% identity to srs:SerAS12_0641)

MetaCyc: 84% identical to 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Escherichia coli K-12 substr. MG1655)
RXN-24043 [EC: 1.17.7.4]; 1.17.7.4 [EC: 1.17.7.4]

Predicted SEED Role

"4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis (EC 1.17.1.2)

MetaCyc Pathways

• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (11/12 steps found)
• isoprene biosynthesis I (9/10 steps found)
• taxadiene biosynthesis (engineered) (11/13 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.1.2 or 1.17.7.4

Protein Sequence (317 amino acids)

>IAI46_03120 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (Serratia liquefaciens MT49)
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEI
GEVPDGSILIFSAHGVSQAVRAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILI
GHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDESNLCFMTQTTLSVDDTSDVID
ALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
KPAYLIDSAADIQEAWLKNASNIGVTAGASAPDVLVQDVIARLKALGGLNVHEVSGREEN
IVFEVPKELRVDIRQVD