Protein Info for IAI46_03100 in Serratia liquefaciens MT49

Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 PF06574: FAD_syn" amino acids 12 to 167 (156 residues), 195.4 bits, see alignment E=8.5e-62 TIGR00083: riboflavin biosynthesis protein RibF" amino acids 18 to 306 (289 residues), 379.5 bits, see alignment E=1.2e-117 TIGR00125: cytidyltransferase-like domain" amino acids 18 to 53 (36 residues), 31.1 bits, see alignment 2.1e-11 PF01467: CTP_transf_like" amino acids 19 to 170 (152 residues), 31.9 bits, see alignment E=2.2e-11 PF01687: Flavokinase" amino acids 184 to 306 (123 residues), 147.3 bits, see alignment E=4.1e-47

Best Hits

Swiss-Prot: 76% identical to RIBF_SHIFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Shigella flexneri

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 98% identity to spe:Spro_0697)

MetaCyc: 76% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (312 amino acids)

>IAI46_03100 bifunctional riboflavin kinase/FAD synthetase (Serratia liquefaciens MT49)
MELIRGIHNIRARHHGCVLTIGNFDGVHRGHQALLEQLKQEGQRLGLPVIVMIFEPQPLE
MFAADKAPARLTRLRDKAKYLAQAGVDYLLCVKFDPRFAANTAQAFVADLLVEKLGVKFL
MVGDDFRFGATRQGDFPLLQKAGKEYGFEVISTPTFREGDQRISSTAIRNALRDDDLAQA
ETLLGHPYSISGRVVHGDELGRTIGFPTANLPLKRLVAPVKGVYAVEVYGLGPEPLPGVA
NIGTRPTVAGVRQQLEVHLLDVTMDLYGRHIDVVLRAKLRNEQRFASLDALKQQIANDVV
TARKFFGLKTLV