Protein Info for IAI46_02360 in Serratia liquefaciens MT49

Annotation: leucyl aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 PF02789: Peptidase_M17_N" amino acids 19 to 147 (129 residues), 134.3 bits, see alignment E=2.5e-43 PF00883: Peptidase_M17" amino acids 186 to 489 (304 residues), 419.7 bits, see alignment E=7.4e-130

Best Hits

Swiss-Prot: 99% identical to AMPA_SERP5: Probable cytosol aminopeptidase (pepA) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 99% identity to srr:SerAS9_0496)

MetaCyc: 91% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1, 3.4.11.9

Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (503 amino acids)

>IAI46_02360 leucyl aminopeptidase (Serratia liquefaciens MT49)
MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKVGQTL
LLHHVPNILSERILLIGCGKERELDERQYKQVIQKTINTLNDTGSMEAVCFLTELHVKGR
NTYWKVRQAVETAKETLYTFDQLKSNKVEPRRPLRKMVFNVPTRRELTSGERAIQHGLAV
SSGIKAAKDLGNMPPNICNAGYLASQARQLADAFSTNITTRVIGEQQMKELGMNAYLAVG
AGSQNESLMSVMEYKGNPNPDAKPIVLVGKGLTFDSGGISIKPADSMDEMKYDMCGAATV
YGVMRVVAELNLPLNVIGVLAGCENMPGGSAYRPGDVLTTMSGQTVEVLNTDAEGRLVLC
DTLTYVERFDPELVIDVATLTGACVIALGHHITGLMSNHNPLAHELIGASEQAGDRAWRL
PMSDEYYEQLDSNFADMANIGGRPGGAITAACFLSRFTRKYSWAHLDIAGTAWRSGKAKG
ATGRPVAMLAQFLLNRAGLNGDD