Protein Info for IAI46_02350 in Serratia liquefaciens MT49

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 958 transmembrane" amino acids 510 to 530 (21 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 10 to 938 (929 residues), 1282.6 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 21 to 638 (618 residues), 544 bits, see alignment E=5.3e-167 PF09334: tRNA-synt_1g" amino acids 48 to 110 (63 residues), 30.9 bits, see alignment 3.1e-11 PF08264: Anticodon_1" amino acids 681 to 832 (152 residues), 157.2 bits, see alignment E=7.2e-50 PF10458: Val_tRNA-synt_C" amino acids 893 to 953 (61 residues), 67.4 bits, see alignment 2.7e-22

Best Hits

Swiss-Prot: 86% identical to SYV_ECOLI: Valine--tRNA ligase (valS) from Escherichia coli (strain K12)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 75% identity to asa:ASA_3821)

MetaCyc: 86% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (958 amino acids)

>IAI46_02350 valine--tRNA ligase (Serratia liquefaciens MT49)
MEKTNSHLDTAYDPKEIEQKLYDHWENQGYFKPNGDTSQESFCIMIPPPNVTGSLHMGHA
FQQTIMDTMIRYQRMQGKNTLWQAGTDHAGIATQMVVERKIAAEENKTRHDYGRDAFIDK
IWQWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVREVFVRLHKEDLIYRGKRLV
NWDPKLRTAISDLEVENRESKGSMWHLRYPLADGAKTAEGKDYLVVATTRPETVLGDTGV
AVNPEDPRYKDLIGKEIILPLVGRRIRIVGDEHADMEKGTGCVKITPAHDFNDYEVGKRH
GLPMINILTFDGDIRQEAEVFNTLGEICTDYCNEIPAQFRGLERFAARKAVVAAFDELGL
LDEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRTAPLAKVAVEAVEQGEIQFVPKQYENMY
FSWMRDIQDWCISRQLWWGHRIPAWYDVNGKVYVGRSEEEVRSENNLGADVVLTQDEDVL
DTWFSSGLWTFSTLGWPEQTDALKTFHPTSVMVSGFDIIFFWIARMIMLTMHFMKDENGK
PQVPFKTVYMTGLIRDDEGQKMSKSKGNVIDPLDMVDGISLEDLLEKRTGNMMQPQLAEK
IRKRTEKQFPNGIEPHGTDALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVL
MNAEGQDCGFNGGEKVLSLADRWILAEFNRTVKAYREALDTYRFDLAANILYEFTWNQFC
DWYLELTKPVVSNGSEAEQRGTRHTLITVLEALLRLAHPVIPFITETIWQRVKTLTGETA
DTIMLQPFPEYDAALEDAQALNDLEWIKQAITAVRNIRAEMNIAPSKQLEVLLRNCSADA
QRRVQENQSFIERLARLESIALLPAGDKGPVSVTKLVDGAELLIPMAGFIDKDAEIARLS
KEMGKLDAEIASIEGKLANEGFVARAPEAVVAKERERLAACKEGKVKLQEQQATIAAL