Protein Info for IAI46_02265 in Serratia liquefaciens MT49

Annotation: 2-iminobutanoate/2-iminopropanoate deaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00004: reactive intermediate/imine deaminase
  • amino acids 3 to 126 (124 residues), 175.3 bits, E=2.7e-56
• PF01042: Ribonuc_L-PSP
  • amino acids 8 to 126 (119 residues), 152.1 bits, E=3.3e-49

Best Hits

Swiss-Prot: 91% identical to RIDA_SALTY: 2-iminobutanoate/2-iminopropanoate deaminase (ridA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: None (inferred from 97% identity to srr:SerAS9_0484)

MetaCyc: 91% identical to 2-iminobutanoate/2-iminopropanoate deaminase monomer (Salmonella enterica enterica serovar Typhimurium str. LT2)
Tryptophanase. [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2]; 3.5.99.10,4.3.1.-,4.3.1.19,4.4.1.1,4.4.1.1,4.4.1.2,3.5.99.7,4.4.1.11 [EC: 3.5.99.10, 4.1.99.1, 4.1.99.2, 4.3.1.13, 4.3.1.17, 4.3.1.18, 4.3.3.8, 4.4.1.1, 4.4.1.13, 4.4.1.15, 4.4.1.25, 4.4.1.28, 4.4.1.35, 4.5.1.2, 3.5.99.7, 4.3.1.19, 4.4.1.11, 4.4.1.2]

Predicted SEED Role

"Bona fide RidA/YjgF/TdcF/RutC subgroup"

MetaCyc Pathways

• aspartate superpathway (25/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-threonine degradation I (6/6 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• homocysteine and cysteine interconversion (4/4 steps found)
• D-serine degradation (3/3 steps found)
• L-cysteine degradation II (3/3 steps found)
• L-methionine degradation II (3/3 steps found)
• L-serine degradation (3/3 steps found)
• glycine degradation (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-cysteine biosynthesis III (from L-homocysteine) (2/2 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (4/5 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (5/7 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• seleno-amino acid detoxification and volatilization III (2/3 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (4/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• L-threonine degradation V (1/2 steps found)
• dimethyl sulfide biosynthesis from methionine (1/2 steps found)
• seleno-amino acid detoxification and volatilization I (1/2 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• glycine betaine degradation I (5/8 steps found)
• glycine betaine degradation III (4/7 steps found)
• sulfolactate degradation III (1/3 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• superpathway of sulfolactate degradation (2/6 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Propanoate metabolism
• Selenoamino acid metabolism
• Sulfur metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.17, 4.3.1.18, 4.3.1.19, 4.4.1.1, 4.4.1.11, 4.4.1.15

Use Curated BLAST to search for 3.5.99.10 or 3.5.99.7 or 4.1.99.1 or 4.1.99.2 or 4.3.1.13 or 4.3.1.17 or 4.3.1.18 or 4.3.1.19 or 4.3.3.8 or 4.4.1.1 or 4.4.1.11 or 4.4.1.13 or 4.4.1.15 or 4.4.1.2 or 4.4.1.25 or 4.4.1.28 or 4.4.1.35 or 4.5.1.2

Protein Sequence (128 amino acids)

>IAI46_02265 2-iminobutanoate/2-iminopropanoate deaminase (Serratia liquefaciens MT49)
MSRNISTELAPAAIGPYVQGVDLGSMIITSGQIPVDPKTGAVADDVAAQARQSLENVKAI
VEAAGLKVADIVKTTVFVKDLNDFATVNAAYEAFFTEHSAPFPARSCVEVARLPKDVKIE
IEAIAVRR