Protein Info for IAI46_02135 in Serratia liquefaciens MT49

Annotation: anaerobic ribonucleoside-triphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 PF03477: ATP-cone" amino acids 4 to 89 (86 residues), 73.1 bits, see alignment E=3.4e-24 TIGR02487: anaerobic ribonucleoside-triphosphate reductase" amino acids 115 to 705 (591 residues), 672.2 bits, see alignment E=3.2e-206 PF13597: NRDD" amino acids 116 to 705 (590 residues), 645.4 bits, see alignment E=1.3e-197 PF01228: Gly_radical" amino acids 581 to 686 (106 residues), 105.4 bits, see alignment E=3.5e-34

Best Hits

Swiss-Prot: 87% identical to NRDD_SALTY: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 89% identity to ddc:Dd586_0360)

MetaCyc: 86% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (712 amino acids)

>IAI46_02135 anaerobic ribonucleoside-triphosphate reductase (Serratia liquefaciens MT49)
MKPVVIKRDGCQVPFDEARIGQAIERAALAVGIVDADYCATVARVVAQQISQQPRVDIHE
IQRAVENQLMAGEYKQLARAYIEYRHDRDVARELRGRLNQEIRGLVEQSNVALLNENANK
DSKVIPTQRDLLAGIVAKHYAKQHILPRDVVLAHERGEIHYHDLDYSPFFPMFNCMLIDL
KGMLTNGFKMGNAEIEPPKSISTATAVTAQIIAQVASHIYGGTTINRIDEILAPFVTESF
NKHQQVAEQWQIPDSQGYAMARTEKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTS
WESRLIQRSILSNRIAGLGKNRKTAVFPKLVFAIRDGLNHQYGDPNYDIKQLALECASKR
MYPDILNYDQVVKVTGSFKTPMGCRSFLGTYEEEGELVHDGRNNIGVISLNLPRIALEAM
GDESRFWDLLDQRLLLAKKALMTRIARLEGIKARVAPILYMEGACGVRLKADDNIAEIFK
NGRASISLGYIGLHETINALFGNEQHVYDDETLRAKAIAIVAHLRAATESWKAETGYGFS
LYSTPSENLCDRFCRLDTADFGVVSGVTDKGYYTNSFHLDVEKKVNPYDKLDFEAPYPPL
ANGGFICYGEYPNLQHNLKALEDVWDYSYSRVPYYGTNTPIDECYECGFTGEFSCTSKGF
TCPKCGNHEPSKVSVTRRVCGYLGSPDARPFNAGKQEEVKRRVKHLGNGQID