Protein Info for IAI46_01875 in Serratia liquefaciens MT49

Annotation: serine-type D-Ala-D-Ala carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF02113: Peptidase_S13" amino acids 22 to 468 (447 residues), 553.8 bits, see alignment E=2.8e-170 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 46 to 447 (402 residues), 556.4 bits, see alignment E=1.6e-171 PF13354: Beta-lactamase2" amino acids 289 to 442 (154 residues), 24.8 bits, see alignment E=1.4e-09

Best Hits

Swiss-Prot: 80% identical to DACB_ECOLI: D-alanyl-D-alanine carboxypeptidase DacB (dacB) from Escherichia coli (strain K12)

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 99% identity to srs:SerAS12_0419)

MetaCyc: 80% identical to peptidoglycan DD-endopeptidase DacB (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]; 3.4.-.- [EC: 3.4.16.4]

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>IAI46_01875 serine-type D-Ala-D-Ala carboxypeptidase (Serratia liquefaciens MT49)
MRFSRIVSALACAFVLNANAAPVEDYTQYLPDGANLALVVQKIGASAPTIDYHSQQMALP
ASTQKVLTALAALLQLGPDYRFTTTLESQGDISDGVLRGNLIARFGGDPTFKRQSLRNMV
AILKKQGVRQITGDVLVDTSVFASHDKAPGWPWNDLTQCFSAPPAAAIVDRNCFSVSLYS
APNPGDMAFIRVASYYPVNMFSQVRTLAKGSADAQYCELDVVPGELNRFTLTGCLTQRSD
PLPLAFAIQDGASYAGAILKDELTQAGIQIDGHLKRQTQPGLKGTVIAQTQSAPLHELLK
IMLKKSDNMIADTVFRTIGHERFGVPGTWRAGADAVRQVLRQKAGVDLGNSIVVDGSGLS
RHNLLAPATMMQALQYIAQHDSELNFISMLPLSGYDGTLRYRGGLHEAGVDGKVSAKTGA
LQGVYNLAGFITTASGQRFAFVQYLSGYAVPPEDQKQRRAPLVRFESRLYRDIYQNN