Protein Info for IAI46_01435 in Serratia liquefaciens MT49

Annotation: trimethylamine-N-oxide reductase TorA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 844 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details transmembrane" amino acids 378 to 403 (26 residues), see Phobius details TIGR02164: trimethylamine-N-oxide reductase TorA" amino acids 9 to 829 (821 residues), 1475.5 bits, see alignment E=0 PF18364: Molybdopterin_N" amino acids 49 to 89 (41 residues), 61.5 bits, see alignment 9.1e-21 TIGR00509: molybdopterin guanine dinucleotide-containing S/N-oxide reductases" amino acids 50 to 829 (780 residues), 1199.8 bits, see alignment E=0 PF00384: Molybdopterin" amino acids 93 to 566 (474 residues), 260.5 bits, see alignment E=3.5e-81 PF01568: Molydop_binding" amino acids 684 to 805 (122 residues), 83.7 bits, see alignment E=1.4e-27

Best Hits

Swiss-Prot: 82% identical to TORA_ECOLI: Trimethylamine-N-oxide reductase 1 (torA) from Escherichia coli (strain K12)

KEGG orthology group: K07811, trimethylamine-N-oxide reductase (cytochrome c) 1 [EC: 1.7.2.3] (inferred from 82% identity to eum:ECUMN_1179)

MetaCyc: 82% identical to trimethylamine N-oxide reductase 1 (Escherichia coli K-12 substr. MG1655)
Trimethylamine-N-oxide reductase (cytochrome c). [EC: 1.7.2.3]; RXN0-5264 [EC: 1.7.2.3]

Predicted SEED Role

"Trimethylamine-N-oxide reductase (EC 1.6.6.9)" in subsystem trimethylamine N-oxide (TMAO) reductase (EC 1.6.6.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.6.9, 1.7.2.3

Use Curated BLAST to search for 1.6.6.9 or 1.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (844 amino acids)

>IAI46_01435 trimethylamine-N-oxide reductase TorA (Serratia liquefaciens MT49)
MKSSQKVSLSRRRFLAQLGGLTAIGMIGPSLLVPKNALAQGGLQEGILTGSHWGAIRATV
VDGRFVAAKPFEMDKYPSKMIAGLPDHVHGTARIRYPMVRIDWMRKRHLSDTTQRGDNRF
VRVSWDEALNLFYQELERVQNTYGPSALLSGTGWQSTGMFHNAAGMLARALALHGDSVGT
GGDYSTGAAQVILPRVVGSMEVYEQQTSWPLVLQNSKTLVLWGSDLVKNQQANWWCPDHD
VYEYYAELKAKVAKGEINVVSIDPVVSSTHAFLGREHVQHIALNPQTDVPLQLALAHTLY
SENLHDTHFLKTYCVGFEQFLPYLLGQTDGQPKDAQWASALCGIDAEIIRALARQMAANR
TQIIAGWCVQRMQHGEQWAWMTVVLAAMLGQIGLPGGGFGFGWHYNGAGTPSRKGVILSG
FSGSTATPARHAKGDFKGYSSTIPIARFIDAMLEPGKEIDWNGKRVKLPMLKMCVFAGTN
PFHRHQQVNRMIAGWQKLETVISIDNQWTSTCRFADIVLPATTQFERNDLDQFGNHSNRG
IIAMKQLVPPQFEARNDFDILRELCKLFDRERDFTEGLDEMGWIKRIYQDGARQGKGRGV
TLPAFDEFWDGDGYVEFHHPEMFVRHQAFRQDPDLEPLGTPSGLIEIYSKSIADMNYKNC
KGYPMWFEKAERSHGGPLSASYPLHLQSVHPDFRLHSQLCESETLRAQYSVAGKEPVFIS
PEDADARGINNGDVVRVFNARGQVLAGAVVSDRYAPGVIRIHEGAWYDPERGGVAGSLCK
YGNPNVLTLDIGSSELAQATSAHTALVEIERFNGKIEPVTAFDGPLEMVAQCDYVPMTPA
NVPQ