Protein Info for IAI46_01330 in Serratia liquefaciens MT49

Annotation: NAD(+) diphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF09297: zf-NADH-PPase
  • amino acids 93 to 124 (32 residues), 33.3 bits, 3.2e-12
• PF00293: NUDIX
  • amino acids 130 to 239 (110 residues), 66.8 bits, E=2e-22

Best Hits

Swiss-Prot: 84% identical to NUDC_YERE8: NADH pyrophosphatase (nudC) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K03426, NAD+ diphosphatase [EC: 3.6.1.22] (inferred from 98% identity to spe:Spro_0286)

MetaCyc: 77% identical to RNA decapping hydrolase (Escherichia coli K-12 substr. MG1655)
NAD(+) diphosphatase. [EC: 3.6.1.22]; RXN0-7346 [EC: 3.6.1.22]

Predicted SEED Role

"NADH pyrophosphatase (EC 3.6.1.22)" in subsystem Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.22)

MetaCyc Pathways

• NAD salvage pathway II (PNC IV cycle) (5/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
• NAD salvage (plants) (6/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.22

Protein Sequence (255 amino acids)

>IAI46_01330 NAD(+) diphosphatase (Serratia liquefaciens MT49)
MELALTGNENGWWIVSHESKLWLPNGELPCGLAASFSLQGRMARAIGEWEGLPVWLVRQA
MPQDMGSVRQLLELDRGLFQLAGRGVQLADFYRSHRYCGYCGHEMHLSRTESACLCGNCR
ERYYPQIAPCVIVAIRRDDEILLAQHVRHRGGIHTVLAGFVEVGETLEQAAAREVMEESN
IEIKNLRYVTSQPWPFPHSLMMAFMADYHRGEIRHDPKELLNAGWYRYDRLPLLPPPGTV
ARRLIEDTVALCREQ