Protein Info for IAI46_01320 in Serratia liquefaciens MT49

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 8 to 90 (83 residues), 104.8 bits, E=1.6e-34
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 129 to 581 (453 residues), 742.7 bits, E=5.6e-228
• PF01964: ThiC_Rad_SAM
  • amino acids 130 to 577 (448 residues), 621.1 bits, E=8.8e-191

Best Hits

Swiss-Prot: 88% identical to THIC_YERPE: Phosphomethylpyrimidine synthase (thiC) from Yersinia pestis

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 91% identity to pct:PC1_0216)

MetaCyc: 89% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (627 amino acids)

>IAI46_01320 phosphomethylpyrimidine synthase ThiC (Serratia liquefaciens MT49)
MDTLQGVSFPNSRRIYLQGSRNDLQVPMREIQLSPTLLGGDKDNPQYQPNEAIPVYDTAG
PYGDPQSELNVHTGLVKLRAGWIAERGDTELLNGVSSGFTQQRLADEGLDHLRFEHLPRP
RKAQPGKCVTQLHYARAGVITPEMEFIAIRENMGRERIRGDVLRQQHPGQSWGANLPENI
TPEFVRQEVAAGRAIIPANINHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVW
STRWGADTVMDLSTGRYIHETREWILRNSPVPIGTVPIYQALEKVNGVAENLTWEMFRDT
LLEQAEQGVDYFTIHAGVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFRE
ICEICAAYDVSLSLGDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHV
PMQMIRRNMTEELEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTP
KEHLGLPNKEDVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALD
PATARAYHDETLPQESGKIAHFCSMCGPKFCSMKISQEVRDYAAAQEAAKPIEVQLTGME
KMSAEFRSRGSELYHSAGNLQEELSND