Protein Info for IAI46_01035 in Serratia liquefaciens MT49

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 10 to 317 (308 residues), 429.8 bits, E=7.6e-133
  • amino acids 553 to 692 (140 residues), 28.1 bits, E=1.5e-10
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 13 to 761 (749 residues), 1138 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 160 to 227 (68 residues), 22.5 bits, E=6.5e-09
  • amino acids 434 to 756 (323 residues), 536.4 bits, E=2.7e-165

Best Hits

Swiss-Prot: 87% identical to METE_PECAS: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 87% identity to eca:ECA0181)

MetaCyc: 84% identical to cobalamin-independent homocysteine transmethylase (Escherichia coli K-12 substr. MG1655)
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase. [EC: 2.1.1.14]

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (25/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• S-adenosyl-L-methionine salvage II (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.14

Use Curated BLAST to search for 2.1.1.14

Protein Sequence (763 amino acids)

>IAI46_01035 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase (Serratia liquefaciens MT49)
MGNMTMAILNHTLGFPRVGLRRELKKAQESYWAGNSTQEELLAVGRELRARHWQQQQQAG
VDLVPVGDFAWYDHVLTTSLLLGNVPARHQNADGSIDLDTLFRIGRGRAPTGKPAAAAEM
TKWFNTNYHYMVPEFHKGQQFKLGWTQLLEEVDEALALGHKIKPVLLGPVTYLWLGKVKG
EAFDRLSLLKDILPVYQQVLAELAQRGVEWVQIDEPALVLELPQEWQNAFKPAYEALQGQ
VKLLLTTYFDSVGHNLDTVRELPVQGLHVDLVAGHDDIDALNKALPTEWLLSLGVINGRN
VWRADLSSWFERLQPLVGSRPLWIGTSCSLLHSPIDLSVETRLDEEVKSWFAFALQKCAE
LALLSAALNAPGAEKQAELDAYSAPIRARRQSSRVHNAQVSQRLAAITAQDSERQRPYAE
RAQAQHARFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGNNYRTSISEHIKQAIVEQ
ERLGLDVLVHGEAERNDMVEYFGENLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAI
TVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVTREVIAKQIALALRDEVEDLEKAG
IGIIQIDEPALREGLPLRRSDWAAYLNWAVEAFKLNAAVAQDDTQIHTHMCYCEFNDIMD
SIAALDADVITIETSRSDMDLLEAFKEFEYPNEIGPGVYDIHSPNVPSVEWIEALLRKAA
QNIPAERLWVNPDCGLKTRGWPETRQSLTNMVLAAQRLREQQV