Protein Info for IAI46_00210 in Serratia liquefaciens MT49

Annotation: formate dehydrogenase-N subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1015 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR01553: formate dehydrogenase-N alpha subunit" amino acids 2 to 1014 (1013 residues), 1605.9 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 45 to 103 (59 residues), 59.6 bits, see alignment 3.5e-20 PF00384: Molybdopterin" amino acids 107 to 590 (484 residues), 114 bits, see alignment E=1.2e-36 PF01568: Molydop_binding" amino acids 893 to 1008 (116 residues), 61.3 bits, see alignment E=1.3e-20

Best Hits

Swiss-Prot: 88% identical to FDOG_ECOLI: Formate dehydrogenase-O major subunit (fdoG) from Escherichia coli (strain K12)

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 71% identity to avn:Avin_03810)

MetaCyc: 79% identical to formate dehydrogenase N subunit alpha (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing" (EC 1.2.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1015 amino acids)

>IAI46_00210 formate dehydrogenase-N subunit alpha (Serratia liquefaciens MT49)
MQVSRRQFFKICAGGMAGTTVAALGFAPEVALAETRQYKLLRARETRNTCTYCSVGCGLL
MYSLGDGAKNAKESIFHIEGDPDHPVNRGALCPKGAGLVDFIHSESRLKYPEYRAPGSDK
WQRISWDDAFTRIAKLMKEDRDANFIKTNEQGVTVNRWLSTGMLCASAASNETGYLSQKF
SRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWVDIKNANLIIVMGGNAAEAHPVG
FRWAMEAKIHNKAKLIVIDPRFTRTASVADFYTPIRSGTDIAFLSGVLLYLMTNNKINRE
YVEAYTNASLLVREDFAFDDGLFSGYDAENRKYDKTTWNYQFDENGFAKRDVTLQDPRCV
WNLLKEHVSRYTPEVVTNICGTPKEDFLQVCEYIAETCVADKTASFLYALGWTQHSVGAQ
NIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSQSLPGYMTLPSDKQPDLET
YLKANTPKALLPGQVNYWGNYPKFFVSLMKSFYGDKAQKDNSWGFDWLPKWDKGYDVLQY
FEMMSQGKVNGYFCQGFNPVASFPNKNKVVASLSKLKFLVTIDPLNTETSNFWQNHGEFN
DVDSSKIQTEVFRLPSTCFAEENGSIVNSGRWLQWHWKGADAPGEALNDGEILAGIFTRL
REMYARDGGAVPEQVLNMTWDYLTPDNPAAEEVAMESNGKALADILDADGKVLAKKGEQL
STFAHLRDDGTTASGCWIFAGSWTPAGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYN
RASADPQGKPWDPKRQLLEWDGAKWGGVDIPDYSAAAPGSDVGPFIMQPEGMGRLFATDK
MAEGPFPEHYEPFETPLGTNPLHPNVVSNPAARVFKDDLAAMGKSDKFPYVGTTYRLTEH
FHYWTKHALLNAIAQPEQFVEIGEKLAEKKGIQHGDTVKVSSNRGYIKAKAVVTKRIRTL
QVHGQEVDTIGIPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKAFLVNVEKV