Protein Info for HUW76_10545 in Fusobacterium nucleatum SB010
Annotation: formate--tetrahydrofolate ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to FTHS_FUSNN: Formate--tetrahydrofolate ligase (fhs) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)
KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 96% identity to fnu:FN2082)MetaCyc: 59% identical to formyltetrahydrofolate synthetase subunit (Moorella thermoacetica)
Formate--tetrahydrofolate ligase. [EC: 6.3.4.3]
Predicted SEED Role
"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)
MetaCyc Pathways
- L-histidine degradation III (6/6 steps found)
- superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
- formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
- tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
- folate polyglutamylation (4/5 steps found)
- formaldehyde oxidation VII (THF pathway) (3/4 steps found)
- folate transformations III (E. coli) (6/9 steps found)
- folate transformations II (plants) (7/11 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
- folate transformations I (6/13 steps found)
- reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
- purine nucleobases degradation I (anaerobic) (4/15 steps found)
- purine nucleobases degradation II (anaerobic) (10/24 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (16/56 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.4.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (544 amino acids)
>HUW76_10545 formate--tetrahydrofolate ligase (Fusobacterium nucleatum SB010) MTDIQIAQAAKKENIVEIAKKLGLTEDDIEQYGKYKAKVNLDVLQKSKRPNGKLILVTAI TPTPAGEGKSTVTIGLTQALNKMGKLSAAAIREPSLGPVFGMKGGAAGGGYAQVVPMEDI NLHFTGDMHAIGVAHNLISACIDNHINSGNALKIDITKITWKRVVDMNDRALRNIVIGLG GKANGYPRQDSFQITVGSEIMAILCLSNSITELKEKIKNIVFATSLEGKLLRVGDLHIEG AVTALLKDAIKPNLVQTLENTPVFIHGGPFANIAHGCNSILATKMALKLTDYAVTEAGFA ADLGAEKFIDIKCRLGGIKPDCAVIVATVRALEHHGKGDLKAGLENLDKHIDNIKNKYKL PLVVAINKFITDTDEQIDMIEKFCNERGAEVSLCEVWAKGGEGGIDLAEKVLKAIDNNKV EFDYFYDENLTIKEKIEKICKEIYGADGVVFAPATKKVFDVIEAEGLNKLPVCMSKTQKS ISDNPALLGKPTGFKVTINELRLAAGAGFIIAMAGNIIDMPGLPKKPSAEVIDIDENGVI SGLF