Protein Info for HUW76_10470 in Fusobacterium nucleatum SB010

Annotation: asparagine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00457: asparagine--tRNA ligase" amino acids 2 to 461 (460 residues), 632.1 bits, see alignment E=2.7e-194 PF01336: tRNA_anti-codon" amino acids 21 to 96 (76 residues), 45.3 bits, see alignment E=6.8e-16 PF00152: tRNA-synt_2" amino acids 118 to 455 (338 residues), 234.7 bits, see alignment E=1.3e-73

Best Hits

Swiss-Prot: 94% identical to SYN_FUSNN: Asparagine--tRNA ligase (asnS) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K01893, asparaginyl-tRNA synthetase [EC: 6.1.1.22] (inferred from 94% identity to fnu:FN0040)

Predicted SEED Role

"Asparaginyl-tRNA synthetase (EC 6.1.1.22)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>HUW76_10470 asparagine--tRNA ligase (Fusobacterium nucleatum SB010)
MITVKDIFRHGEDYLNKEIELFGWVRKIRDQKKFGFIELNDGSFFKGVQIVFEEGLENFD
EVSRLSISSTIKVKGTLVKSQGSGQDLEVKADKIEIFQKADLEYPLQNKRHTFEYLRTKA
HLRPRTNTFSAVFRVRSVLAYALHKFFQENNFVYVHTPIITGSDAEGAGEMFRVTTLDMN
KLPKKENGEVDFTKDFFGKSTNLTVSGQLNVETFCAAFRNVYTFGPTFRAEYSNTARHAS
EFWMVEPEIAFADLSANMELAEAMVKFIIKYVMDTCPEEMEFFNSFIEKGLFDKLNNVLN
NEFGRITYTEAIEILEKSGKKFEFPVKWGIDLQSEHERYLAEEYFKKPVFVTDYPKEIKA
FYMKLNEDGRTVRAMDLLAPGIGEIIGGSQREDSYEILLKRMKELGLNEEDYEFYLDLRR
FGSFPHSGYGLGFERMMMYLTGMQNIRDVIPFPRTPNNAEF