Protein Info for HUW76_08820 in Fusobacterium nucleatum SB010

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00233: tryptophan--tRNA ligase
  • amino acids 2 to 320 (319 residues), 309.1 bits, E=1.9e-96
• PF00579: tRNA-synt_1b
  • amino acids 4 to 275 (272 residues), 211.1 bits, E=1.1e-66

Best Hits

Swiss-Prot: 96% identical to SYW_FUSNN: Tryptophan--tRNA ligase (trpS) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 96% identity to fnu:FN0405)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

• tRNA charging (20/21 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Tryptophan metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Protein Sequence (325 amino acids)

>HUW76_08820 tryptophan--tRNA ligase (Fusobacterium nucleatum SB010)
MKRSLSGIQPSGILHIGNYFGAMKQFIDLQDDYDGFYFIADYHSLTSLTKAETLKENTYN
IVLDYLAIGLDPNKSTIFLQSNVPEHTELTWLLSNITPVGLLERGHSYKDKIAKGIPSNT
GLLTYPVLMAADILIYDSDIVPVGKDQKQHLEMTRDIAMKFNQQYEVEFFKLPEPLILDD
SAIVPGTDGQKMSKSYNNTINMFATKKKLKEQVMSIVTDSTPLEEPKNPDNNIAKIYALF
NNIDKQNELKEKFLAGNFGYGHAKTELLNSILEYFGKAREKREELEKDIDYVKDVLNEGS
KKARAIAIEKIKKAKEIVGLIGNIY