Protein Info for HUW76_08760 in Fusobacterium nucleatum SB010

Annotation: dihydroorotase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00857: dihydroorotase, multifunctional complex type
  • amino acids 13 to 419 (407 residues), 436.1 bits, E=6.7e-135
• PF01979: Amidohydro_1
  • amino acids 47 to 415 (369 residues), 95.6 bits, E=5.6e-31
• PF12890: DHOase
  • amino acids 47 to 233 (187 residues), 81.5 bits, E=8.9e-27

Best Hits

Swiss-Prot: 95% identical to PYRC_FUSNN: Dihydroorotase (pyrC) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K01465, dihydroorotase [EC: 3.5.2.3] (inferred from 95% identity to fnu:FN0420)

MetaCyc: 55% identical to dihydroorotase (Bacillus subtilis subtilis 168)
Dihydroorotase. [EC: 3.5.2.3]

Predicted SEED Role

"Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis (EC 3.5.2.3)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• UMP biosynthesis I (5/6 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (7/9 steps found)
• superpathway of histidine, purine, and pyrimidine biosynthesis (30/46 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.3

Protein Sequence (425 amino acids)

>HUW76_08760 dihydroorotase (Fusobacterium nucleatum SB010)
MLLKNCKILKNSKFEKVDILIKDDKIEKISENINVTDENTIDVKNRFVTAGFIDAHVHWR
EPGFSKKETVYTASRAAARGGFTTVMTMPNLNPVPDSVETLNKQLEIIKNDSVIRAIPYG
AITKEEYGRELSDMEAIANDVFAFTDDGRGIQSANVMYEAMLMGAKLNKAIVAHCEDNSL
IRGGAMHEGKRSVELGIKGIPSICESTQIARDILLAEAAKCHYHVCHISAKESVRAVREG
KQNGIKVTCEVTPHHLLSCDEDIKEDNGMWKMNPPLRAREDRNALIVGILDGTIDIIATD
HAPHTMEEKIRGIEKSSFGIVGSETAFAQLYTKFVKTDIFSLEMLVKLMSENVAKIFDLP
YGKLEENSFADIVVVDLEKEITINPSNFLSKGKNTPYINEKINGIPVLTISNGKIAYIDK
EEINL