Protein Info for HUW76_08740 in Fusobacterium nucleatum SB010

Annotation: dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 305 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 5 to 303 (299 residues), 386 bits, see alignment E=5.3e-120 PF01180: DHO_dh" amino acids 5 to 287 (283 residues), 340.6 bits, see alignment E=1.5e-105 PF03060: NMO" amino acids 213 to 268 (56 residues), 30.8 bits, see alignment E=4.3e-11

Best Hits

Swiss-Prot: 98% identical to PYRDB_FUSNN: Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit (pyrD) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 98% identity to fnu:FN0424)

MetaCyc: 68% identical to dihydroorotate dehydrogenase (catalytic subunit) (Bacillus subtilis subtilis 168)
Orotate reductase (NADH). [EC: 1.3.1.14]

Predicted SEED Role

"Dihydroorotate dehydrogenase, catalytic subunit (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1

Use Curated BLAST to search for 1.3.1.14 or 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (305 amino acids)

>HUW76_08740 dihydroorotate dehydrogenase (Fusobacterium nucleatum SB010)
MSERLRVQIPGLDLKNPIMPASGCFAFGIEYAELYDISKLGAIMIKAATKEARFGNPTPR
VAETSSGMLNAIGLQNPGVDEIISNQLKKLEKYDVPIIANVAGSDIDDYVYVADKISKSP
NVKALELNISCPNVKHGGIQFGTDPNVARNLTEKVKAVSSVPVYVKLSPNVTDIVAMAKA
VETGGADGLTMINTLVGIVLDRKTGKPIIANTTGGLSGPAIKPVAIRMVYQVAQAVNIPI
IGMGGVMDEWDVIDFISAGASAVAVGTANFTDPFVCPKIIDNLESTLDELGVNHILDLKG
RAFRY