Protein Info for HUW76_08290 in Fusobacterium nucleatum SB010

Annotation: cadmium-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 614 transmembrane" amino acids 7 to 40 (34 residues), see Phobius details amino acids 62 to 87 (26 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details amino acids 258 to 283 (26 residues), see Phobius details amino acids 562 to 582 (21 residues), see Phobius details amino acids 588 to 609 (22 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 62 to 607 (546 residues), 567.3 bits, see alignment E=6.5e-174 TIGR01512: cadmium-translocating P-type ATPase" amino acids 62 to 610 (549 residues), 514.5 bits, see alignment E=4.3e-158 TIGR01494: HAD ATPase, P-type, family IC" amino acids 82 to 592 (511 residues), 243.8 bits, see alignment E=4.1e-76 PF00122: E1-E2_ATPase" amino acids 112 to 291 (180 residues), 176 bits, see alignment E=5.7e-56 PF00702: Hydrolase" amino acids 308 to 519 (212 residues), 90.7 bits, see alignment E=1.7e-29

Best Hits

Swiss-Prot: 48% identical to ATZN_SYNY3: Zinc-transporting ATPase (ziaA) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 89% identity to fnu:FN0258)

Predicted SEED Role

"Cadmium-transporting ATPase (EC 3.6.3.3)" in subsystem Cobalt-zinc-cadmium resistance (EC 3.6.3.3)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (614 amino acids)

>HUW76_08290 cadmium-translocating P-type ATPase (Fusobacterium nucleatum SB010)
MKKKKEVIIVISAILFVIALLVKVNLTLQLILMLIAYILVGKDTVLGAIKNVEKGDFLDE
KFLMTIATLGAILIGEYPEAVAVMLFYEVGELFQGYAINKSRKSIADMMDIKPEYANVIR
NNKSQKVDPDEVQIGETIEIKPGERVPLDATVVKGESTLDTSALTGESVPVEVREGANIL
SGCININGLIIAEVTKEYLDSTVNKVLDLVENASAKKSKSERLITRFSKIYTPIVIGLAV
LLAILPPIISGEYNFRVWIFRALSFLVVSCPCAFVISVPLSFFSGIGAASRAGVLIKGGN
YLEALSKVDTVVFDKTGTLTKGVFNVQKVVVLDKNIDENKFISLVAAAESGSNHPISKSI
QKYYNKEIDTNSINSIKEISGKGIEAIIDNRKILVGNEKLINIPKDISIDDIGTILYVEI
DNKFAGYIVISDELKKDSKKTIKDLKDIGIKKTIMLTGDLEKVSKKVGKDLDLDEVYTNL
LPQDKVSKFEEIIENKKSKGNVVFVGDGINDAPVLARADVGIAMGAMGSDAAIEAADVVI
MTDEPSKIVTAIKSSKKTMKIAMQNITLAFGIKAIVLILSALGIADMWMAVFADTGVTIL
AVLNSFRALKIENN