Protein Info for HUW76_08220 in Fusobacterium nucleatum SB010

Annotation: M20/M25/M40 family metallo-hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 PF01546: Peptidase_M20" amino acids 90 to 326 (237 residues), 35.8 bits, see alignment E=8.1e-13 PF07687: M20_dimer" amino acids 170 to 252 (83 residues), 25 bits, see alignment E=1.5e-09

Best Hits

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>HUW76_08220 M20/M25/M40 family metallo-hydrolase (Fusobacterium nucleatum SB010)
MILEKKLINSIEKYKEEAFALNYYLAKNPEISGKEYNSCKKIMEILNKHQIATKEKFSDI
DTSFLGEVIKKENSNINIAILTEYDALPEVGHACGHSASAAISVLAALALKDNEEYISAN
IDIIGTPDEEDIGMKVTMADKGVFDKYDCAIMVHLGIKNIPNWKMLAFETYEIEFTGFPA
HTAVAPWCGRSALDGLMLSIHAFDLMRKCTKRNTIIEGFIQEGGSATNIIPNKAKAKYTF
RSDSLKYLKEEIIPWFKNIIEGCSLATQTKSKIEIFGNSFSDMNYLKSGTQIIMNVMKDY
GMEYETMDYPEGSSDMGNVSYRCPAFHPSVAITNKNIALHTKEFADIVASEKSEPCIING
ATVIDGFLARMLENSEILKSIKQEFLKNKI