Protein Info for HUW76_08085 in Fusobacterium nucleatum SB010

Annotation: formate C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 3 to 743 (741 residues), 1168.5 bits, E=0
• PF02901: PFL-like
  • amino acids 14 to 602 (589 residues), 696.5 bits, E=4.4e-213
• PF01228: Gly_radical
  • amino acids 619 to 724 (106 residues), 128.9 bits, E=1.1e-41

Best Hits

Swiss-Prot: 64% identical to PFLB_STAAN: Formate acetyltransferase (pflB) from Staphylococcus aureus (strain N315)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 98% identity to fnu:FN0262)

MetaCyc: 58% identical to pyruvate formate-lyase subunit (Clostridium pasteurianum)
Formate C-acetyltransferase. [EC: 2.3.1.54]

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• L-threonine degradation I (6/6 steps found)
• superpathway of N-acetylneuraminate degradation (17/22 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• superpathway of L-threonine metabolism (10/18 steps found)
• mixed acid fermentation (8/16 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.54

Protein Sequence (743 amino acids)

>HUW76_08085 formate C-acetyltransferase (Fusobacterium nucleatum SB010)
MEAWRDFKDGEWKKSINVSDFIKRNYTEYTGDESFLEGPTENTKKLWDILSGMLKIEREK
GIYDAETKIPSKIDAYGAGYIDKNLETIVGLQTDAPLKRAIFPNGGLRMVENSLEAFGYK
LDPTTKEIYEKYRKSHNAGVFSAYTPAIKAARHTGVITGLPDAYGRGRIIGDYRRVALYG
VDRLIEERKREFDAYDPEEMTEDVIRNREEMFEQLEALKALKRMAAAYGFDIGRPAETAQ
EAIQWTYFGYLGAIKDQNGAAMSLGKTAGFLDVYIERDLKEGRITEKQAQEFIDHFIMKL
RIVRFLRTPEYDQLFSGDPVWVTESIGGMNNDGKSWVTKNAFRYLNTLYNLGTAPEPNLT
ILWSERLPENWKKFCSKVSIDTSSLQYENDDIMRPQFGEDYGIACCVSPMAIGKQMQFFG
ARANLPKALLYAINGGKDEIKKDQVTPPGQFERITGDYLDFDEVWEKYDKMLTWLASTYI
KALNIIHYMHDKYSYEALEMALHSLDIKRTEACGIAGLSIVADSLAAIKYGKVRVIRDEA
GDAVDYVVEKPYVPFGNNDDRTDELAVKVVRTFMNKIRSHKMYRDAEPTQSVLTITSNVV
YGKKTGNTPDGRRAGAPFGPGANPMHGRDTRGAVASLASVAKLPFEDANDGISYTFAITP
ETLGKTDDEKKNNLVGLLDGYFKQTGHHLNVNVFGRELLEDAMEHPENYPQLTIRVSGYA
VNFIKLTREQQLDVINRTISNKM