Protein Info for HUW76_07405 in Fusobacterium nucleatum SB010

Annotation: glycerol-3-phosphate 1-O-acyltransferase PlsY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 194 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 48 to 70 (23 residues), see Phobius details amino acids 81 to 100 (20 residues), see Phobius details amino acids 107 to 131 (25 residues), see Phobius details amino acids 137 to 155 (19 residues), see Phobius details amino acids 161 to 177 (17 residues), see Phobius details TIGR00023: acyl-phosphate glycerol 3-phosphate acyltransferase" amino acids 4 to 192 (189 residues), 196.8 bits, see alignment E=1.7e-62 PF02660: G3P_acyltransf" amino acids 9 to 184 (176 residues), 184.5 bits, see alignment E=8.1e-59

Best Hits

Swiss-Prot: 89% identical to PLSY_FUSNN: Glycerol-3-phosphate acyltransferase (plsY) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K08591, glycerol-3-phosphate acyltransferase PlsY [EC: 2.3.1.15] (inferred from 89% identity to fnu:FN0537)

MetaCyc: 41% identical to acyl phosphate:glycerol-3-phosphate acyltransferase (Streptococcus pneumoniae R6)
RXN-9591 [EC: 2.3.1.275]

Predicted SEED Role

"Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15 or 2.3.1.275

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (194 amino acids)

>HUW76_07405 glycerol-3-phosphate 1-O-acyltransferase PlsY (Fusobacterium nucleatum SB010)
MTFFCLIILSYFFGAIPSGVWIGKIFKNIDVRDYGSKNSGATNSYRVLGAKLGIAVLIID
VLKGFIPLYIASKFNLKYNDLVILGLVAILAHTFSCFISFKGGKGVATSLGVFLFLAPVI
TLILLVIFILVVYFTKYISLGSITAAFLLPIFTFFTHRDTYLFTLSVVIAIFVIYRHKTN
ISRLLSGTENKFKF