Protein Info for HUW76_07160 in Fusobacterium nucleatum SB010

Annotation: acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 95% identical to LPXA_FUSNN: Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K00677, UDP-N-acetylglucosamine acyltransferase [EC: 2.3.1.129] (inferred from 95% identity to fnu:FN0595)

MetaCyc: 51% identical to acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase (Vibrio cholerae O1 biovar El Tor str. N16961)
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase. [EC: 2.3.1.129]

Predicted SEED Role

"Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (EC 2.3.1.129)" in subsystem KDO2-Lipid A biosynthesis (EC 2.3.1.129)

MetaCyc Pathways

lipid IV_A biosynthesis (P. gingivalis) (8/9 steps found)
superpathway of (Kdo)₂-lipid A biosynthesis (13/17 steps found)
lipid IV_A biosynthesis (E. coli) (5/6 steps found)
lipid IV_A biosynthesis (H. pylori) (5/6 steps found)
lipid IV_A biosynthesis (P. putida) (5/6 steps found)
lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (5/6 steps found)
lipid IV_A biosynthesis (generic) (5/6 steps found)
superpathway of Kdo₂-lipid A biosynthesis (13/25 steps found)

KEGG Metabolic Maps

Lipopolysaccharide biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (257 amino acids)

>HUW76_07160 acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase (Fusobacterium nucleatum SB010)
MVDIHSTAIIEDGAIIEDGVKIGPYCIVGKDVIIKKGTVLQSHIVVEGITEIGENNTIYS
FVSIGKANQDLKYKGEPTKTIIGNNNSIREFVTIHRGTNDRWETRIGNGNLLMAYVHVAH
DVIVGDDCILANNVTLAGHVVVDSHAIIGGLTPVHQFSRIGSYCMIGGASAVSQDVCPFV
LAAGNTVVLRGLNIVGLRRRGFSDEEISNLKKAYRILFRQSLQLKDALEELEKDFSEDKN
VKYLVDFVKSSDRGIAR