Protein Info for HUW76_04985 in Fusobacterium nucleatum SB010

Annotation: RluA family pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01479: S4
  • amino acids 13 to 59 (47 residues), 27.4 bits, 2.2e-10
• PF00849: PseudoU_synth_2
  • amino acids 96 to 243 (148 residues), 114.6 bits, E=4.9e-37

Best Hits

KEGG orthology group: K06179, ribosomal large subunit pseudouridine synthase C [EC: 5.4.99.12] (inferred from 91% identity to fnu:FN1026)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (304 amino acids)

>HUW76_04985 RluA family pseudouridine synthase (Fusobacterium nucleatum SB010)
MIEYIIDEDYESVRVDRFLRKHLKNINLSEIYKMLRKGKIKVNNKKISQDYRLVLGDIIF
IFLPEIFKEKNEEKFIEISQIRKEKLKQMIVFENDNLFVINKPLGDVVHKGSGHDISLLE
EFRSYYSNNNINFVNRIDKLTSGLVIGAKNIKTAREVAKEIQAGNIVKKYYILVNGKIEK
DNFILENYLKKDEERVIVSDIEKEGYKKSITYFKKIKEYDKYTLLEAELKTGRTHQLRAQ
LNHLGNNIVGDIKYGKNEKEDIMYLFSYYLKIDLYNLEIKLEIPDLFKLNFHKNSSLLAK
FLNR