Protein Info for HUW76_01880 in Fusobacterium nucleatum SB010

Annotation: ribonuclease PH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01966: ribonuclease PH
  • amino acids 3 to 237 (235 residues), 363.2 bits, E=4.7e-113
• PF01138: RNase_PH
  • amino acids 11 to 141 (131 residues), 93.7 bits, E=1.9e-30
• PF03725: RNase_PH_C
  • amino acids 160 to 224 (65 residues), 47.9 bits, E=1.6e-16
• TIGR00042: non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family
  • amino acids 243 to 430 (188 residues), 193.5 bits, E=2.2e-61
• PF01725: Ham1p_like
  • amino acids 244 to 428 (185 residues), 220.5 bits, E=2.7e-69

Best Hits

KEGG orthology group: K01516, nucleoside-triphosphatase [EC: 3.6.1.15] (inferred from 95% identity to fnu:FN1851)

Predicted SEED Role

"Ribonuclease PH (EC 2.7.7.56) / Nucleoside 5-triphosphatase RdgB (dHAPTP, dITP, XTP-specific) (EC 3.6.1.15)" in subsystem Heat shock dnaK gene cluster extended or tRNA processing (EC 2.7.7.56, EC 3.6.1.15)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (7/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (10/14 steps found)
• UTP and CTP dephosphorylation I (2/7 steps found)
• tRNA processing (4/10 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.56 or 3.6.1.15

Protein Sequence (434 amino acids)

>HUW76_01880 ribonuclease PH (Fusobacterium nucleatum SB010)
MLREDGRNFNEERKIKITKNINIYAEGSVLIEVGNTKVICTASVSDKVPSFLRGTGKGWV
TAEYSMLPRATNERNPREASKGKLAGRTVEIQRLIGRALRASIDLEKLGERLITIDCDVI
QADGGTRTTSITGGYIALALAIKKLLKEEILEENPLISNVAAISVGKVNSELMVDLKYSE
DFAAEVDMNVIMNKKGEFIEVQGTGEESTFTRAELNQLLDLAENSIKRLIKLQDKIINQE
NLKIFLATANKHKIDEISDIFSGIENIEILSIKDGIEIPEVIEDGKTFEDNSKKKAVEIS
KFLNMIAIADDSGLCVDALNGEPGVYSARYSGTGDDLKNNEKLINNLQGIENRNAKFVSV
ITLAKPNGETYSFRGEINGKIVDTPRGNTGFGYDPHFYVEEYQKTLAELPELKNKISHRA
KALEKLKEELKNIL