Protein Info for HMPREF1181_RS16265 in Bacteroides stercoris CC31F

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 TIGR00665: replicative DNA helicase" amino acids 26 to 465 (440 residues), 544 bits, see alignment E=1.3e-167 PF00772: DnaB" amino acids 26 to 126 (101 residues), 111.1 bits, see alignment E=3.9e-36 PF13481: AAA_25" amino acids 200 to 378 (179 residues), 30.9 bits, see alignment E=3.1e-11 PF03796: DnaB_C" amino acids 201 to 464 (264 residues), 362.5 bits, see alignment E=1.7e-112

Best Hits

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 95% identity to bhl:Bache_2196)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>HMPREF1181_RS16265 replicative DNA helicase (Bacteroides stercoris CC31F)
MAEQRRTTRTPKSKSPQPVNDYGRIQPQAPELEEAVLGALMIEKDAYSLVSEILRPESFY
EHRHQLIYSAITDLAVNQKPVDILTVKEQLAKRGDLEEVGGPFYITQLSSKVASSAHIEY
HARIIAQKALARELITFTSNIQSKAFDETLDVDDLMQEAEGKLFEISQQNMKKDYTQINP
VIAEAYDLIQKAAARTDGLSGLESGFTKLDKMTSGWQNSDLIIIAARPAMGKTAFVLSMA
KNIAVNFRNPVALFSLEMSNVQLVNRLISNVCEIPSEKIKSGQLADYEWQQLDYKLRDLL
DAPLYVDDTPSLSVFELRTKARRLVREHGVKVIIIDYLQLMNASGMSFGSRQEEVSTISR
SLKGLAKELNIPIIALSQLNRGVENREGEEGKRPQLSDLRESGAIEQDADMVCFIHRPEY
YKIYTSADGTDLRGMAEIIIAKHRNGAVGDVRLRFIGQYTRFQNPEDDMVIPPPTEGMGG
ATFGSRMNAPIGSPSTPPPPSASDYMPQTDNPFGGVGLDGPVPF