Protein Info for HMPREF1181_RS15495 in Bacteroides stercoris CC31F

Annotation: adenosylmethionine--8-amino-7-oxononanoate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 transmembrane" amino acids 21 to 38 (18 residues), see Phobius details TIGR00433: biotin synthase" amino acids 87 to 378 (292 residues), 397.7 bits, see alignment E=3.2e-123 PF04055: Radical_SAM" amino acids 120 to 276 (157 residues), 72.7 bits, see alignment E=6.5e-24 PF06968: BATS" amino acids 288 to 378 (91 residues), 79.5 bits, see alignment E=2.7e-26 TIGR00508: adenosylmethionine-8-amino-7-oxononanoate transaminase" amino acids 389 to 808 (420 residues), 567.8 bits, see alignment E=1.1e-174 PF00202: Aminotran_3" amino acids 401 to 808 (408 residues), 398.7 bits, see alignment E=3.8e-123

Best Hits

Swiss-Prot: 82% identical to BIOAB_BACTN: Biotin biosynthesis bifunctional protein BioAB (bioB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00833, adenosylmethionine-8-amino-7-oxononanoate aminotransferase [EC: 2.6.1.62] (inferred from 81% identity to bth:BT_1442)

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6) / Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62, EC 2.8.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.62 or 2.8.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (813 amino acids)

>HMPREF1181_RS15495 adenosylmethionine--8-amino-7-oxononanoate transaminase (Bacteroides stercoris CC31F)
MKRNQSPAQTIRFRRWSRKSYAAFASIGRCVTIGCLRKNVADSSLRKQDTVRTIPYSIHT
MTIDELKARVLAGIDISPDQAAWLANTADREALYAAAHEITVQCSQHEFDMCSIINAKSG
RCPENCKWCAQSSHYHTQADVYNLLSAEECLEQAQYNERQDVNRFSLVTSGRKPSPKQLE
QLCNTVRYMRRHSSIQLCASLGLLAEEELRALHNAGVTRYHCNLETAPSFFPTLCSTHTQ
EDKLRTLQAARNAGMDICSGGIIGMGETMEQRIEFAFTLKELGVQSIPINLLSPIPGTPL
ENRPSLTEEEVLVTVALFRFINPTAYLRFAGGRSQLSKDAVKKSLYIGINSAIVGDLLTT
LGSKVSDDKKLIEEAGYEFCGSQFDREHLWHPYTSTTHPLPVYKVKQADGATITLESGQT
LIEGMSSWWCAVHGYNHPVLNRAATEQLGKMSHVMFGGLTHDPAIELGKLLLPLVPPSMQ
KIFYADSGSVAVEVALKMAVQYWYAKGQGASSQSTSVKKQNFVTIRSGYHGDTWNAMSVC
DPVTGMHSLFGSALPVRYFVPQPRSRFDGEWNPEDIRPLQETIEQHADELAALILEPVVQ
GAGGMWFYHPQYLREAAKLCREHGLLLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKA
LTGGYMTLSAVLATNEVADTISNHAPGAFMHGPTFMGNPLACAVACASVRLLTSPEYNWQ
EKVARIEAQLKQELEPARQLPQVADVRVLGAIGVIETKRPVDMAWMQRRFVEEGIWVRPF
GKLVYLMPPFIIKPEQLGTLTKGLIKIIKEIRD