Protein Info for HMPREF1181_RS14225 in Bacteroides stercoris CC31F

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 TIGR00459: aspartate--tRNA ligase" amino acids 2 to 580 (579 residues), 723.9 bits, see alignment E=6.7e-222 PF01336: tRNA_anti-codon" amino acids 19 to 102 (84 residues), 60 bits, see alignment E=2.6e-20 PF00152: tRNA-synt_2" amino acids 121 to 555 (435 residues), 399.9 bits, see alignment E=1.2e-123 PF02938: GAD" amino acids 316 to 395 (80 residues), 50.1 bits, see alignment E=4.2e-17

Best Hits

Swiss-Prot: 92% identical to SYD_BACTN: Aspartate--tRNA ligase (aspS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 94% identity to bhl:Bache_2317)

MetaCyc: 47% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (585 amino acids)

>HMPREF1181_RS14225 aspartate--tRNA ligase (Bacteroides stercoris CC31F)
MYRSHTCGELRISDVNKQVTLAGWVQRSRKMGGMTFVDLRDRYGITQLVFNEEVDAELCE
QANRLGREFVIQIVGTVNERFSKNKNIPTGDIEIIVSELNVLNSALTPPFTIEDNTDGGD
DIRMKYRYLDLRRAAVRKNLELRHKMTIEVRKYLDSKGFIEVETPLLIGSTPEGARDFVV
PSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIAKCFRDEDLRADRQPEFTQIDCEMSF
VQQDDVIELFEGMAKYLFKELRGVELTEPFQRMPWADAMKYYGSDKPDLRFGMKFVELMD
IMKGHGFSVFDNAAYIGGICAESAASYTRKQLDALTEFVKKPQIGAKGMVYARVEADGTV
KSSVDKFYTQEVLQEMKAAFGAKPGDLILILSGDDVMKTRKQLNELRLEMGNQLGLRDKN
KFALLWIVDFPMFEWSEEEGRLMAMHHPFTHPKDEDIPLLDTDPAAVRADAYDMVCNGIE
VGGGSIRIHDAQLQAKMFEILGFTPEKAEEQFGFLMNAFKFGAPPHGGLAYGLDRWVSIF
AGLDSIRDCIAFPKNNSGRDVMLDAPSVIDQKQLDELNLIVEVKE