Protein Info for HMPREF1181_RS14170 in Bacteroides stercoris CC31F

Annotation: NAD-dependent DNA ligase LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 665 PF01653: DNA_ligase_aden" amino acids 2 to 312 (311 residues), 383.8 bits, see alignment E=1.9e-118 TIGR00575: DNA ligase, NAD-dependent" amino acids 10 to 660 (651 residues), 791.7 bits, see alignment E=2.6e-242 PF03120: DNA_ligase_OB" amino acids 316 to 390 (75 residues), 109.9 bits, see alignment E=1.3e-35 PF03119: DNA_ligase_ZBD" amino acids 402 to 428 (27 residues), 47.5 bits, see alignment (E = 3.9e-16) PF14520: HHH_5" amino acids 442 to 495 (54 residues), 25.8 bits, see alignment 3.5e-09 amino acids 510 to 556 (47 residues), 27.1 bits, see alignment 1.4e-09 PF12826: HHH_2" amino acids 505 to 568 (64 residues), 88.7 bits, see alignment E=6.6e-29 PF00533: BRCT" amino acids 588 to 664 (77 residues), 51.7 bits, see alignment E=2.5e-17

Best Hits

Swiss-Prot: 88% identical to DNLJ_BACTN: DNA ligase (ligA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 88% identity to bhl:Bache_2297)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (665 amino acids)

>HMPREF1181_RS14170 NAD-dependent DNA ligase LigA (Bacteroides stercoris CC31F)
MTVKEKIDQLRADLHRHNYNYYVLNAPEISDKEFDDRMRELQDLEKEHPEYQDDNSPTMR
VGSDLNKNFTQVAHKYPMLSLGNTYSESEVTDFYDRVKKALNEDFEICCELKYDGTSISL
TYENGKLVRAVTRGDGEKGDDVTDNVKTIRTIPLVLHGDYPELFEIRGEILMPWEVFEEL
NREKEAREEPLFANPRNAASGTLKLQNSAIVASRKLDAYLYYLLGEELPCDGHYENLQAA
AGWGFKTSEHMKKAHSLEEVFEYIRYWDTERKNLPVATDGIVLKVNSMRQQKNLGFTAKS
PRWAIAYKFQAERALTRLNKVTYQVGRTGAVTPVANLDPVQLSGTIVKRASLHNADIIEG
LDLHIGDMVYVEKGGEIIPKITGVDKDARSMLIGEKVKFITHCPECGSKLIRYEGEAAHY
CPNETSCPPQIKGKIEHFISRKAMNIDGLGPETVDMFYRLGLIKNTADLYQLTADDIKNL
DRMGEKSAENIIKGIEASKEVPFERVLFALGIRFVGETVAKKIAKSFNDIDELENANLEK
LINIDEIGEKIAQSILTYFANPLNRELIERLKSTGLQLYRREEDLSGYTDKLAGQSIVIS
GVFTHHSRDEYKELIEKNGGKNVGSISAKTSFILAGENMGPAKLEKAHKLGIKLMSEDEF
LTLIS