Protein Info for HMPREF1181_RS14100 in Bacteroides stercoris CC31F

Annotation: dihydrofolate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 682 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03571: Peptidase_M49" amino acids 240 to 469 (230 residues), 140 bits, see alignment E=4.7e-45 amino acids 474 to 617 (144 residues), 36.5 bits, see alignment E=1e-13

Best Hits

KEGG orthology group: K01277, dipeptidyl-peptidase III [EC: 3.4.14.4] (inferred from 87% identity to bhl:Bache_3162)

Predicted SEED Role

"Dipeptidyl-peptidase III (EC 3.4.14.4)" (EC 3.4.14.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.14.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (682 amino acids)

>HMPREF1181_RS14100 dihydrofolate reductase (Bacteroides stercoris CC31F)
MKKQLISVMVAASLLTACGGAPKTTAKAEKFDYTVEQFADLQILRYRVPEFENLSLKQKE
LVYYLTEAALQGRDILFDQNGKYNLRIRRMLEAVYTGYTGDKTAADFKAMEVYLKRVWFS
NGIHHHYGCEKFVPGFTPEFFKQALLSVDAATLPLAEGQTVEQLYEEVAPVIFDPKVMPK
RVNQAAGEDLVLTSACNYYDGVTQQEAEAFYSSMKDPKDETPVSYGLNSRLVKENGKIQE
KVWKVGGLYGAAIGKIVYWLKKAEGVAENPEQKAVIAKLIEFYETGDLKTFDDYAILWVK
DLDSRIDFVNGFTESYGDPLGMKASWESLVNFKDLEATRRTEIISSNAQWFEDHSPVEKQ
FKKEKVKGVSAKVITAAILAGDLYPSTAIGINLPNANWIRSHHGSKSVTIGNITDAYNKA
AHGNGFNEEFVYSDAELQLIDKYADTTDELHTDLHECLGHGSGKLLPGVDPDALKAYGST
IEEARADLFGLYYVADPKLVELGLTPSADAYKAQFYTYLMNGLMTQLVRIEPGNNVEEAH
MRNRQLIARWVYEKGAADKVVELVKKDGKTYVVINDYEKVRELFGELLAEIQRIKSTGDY
DAARELVESYAVKVDPELHAEVLERYKKLNLAPYKGFVNPKYEAVTDADGNITDVKVTYD
EGYAEQMLRYSKDYSALPSVND