Protein Info for HMPREF1181_RS13640 in Bacteroides stercoris CC31F

Annotation: type I restriction-modification system subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 472 PF12161: HsdM_N" amino acids 11 to 117 (107 residues), 47.8 bits, see alignment E=3.6e-16 PF02384: N6_Mtase" amino acids 127 to 397 (271 residues), 235.4 bits, see alignment E=1.5e-73 PF01170: UPF0020" amino acids 160 to 328 (169 residues), 43.1 bits, see alignment E=6e-15

Best Hits

Swiss-Prot: 95% identical to T1M_BACTN: Probable type I restriction enzyme BthVORF4518P M protein (BT_4518) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K03427, type I restriction enzyme M protein [EC: 2.1.1.72] (inferred from 96% identity to bvu:BVU_4163)

Predicted SEED Role

"Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)" in subsystem Restriction-Modification System (EC 2.1.1.72)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (472 amino acids)

>HMPREF1181_RS13640 type I restriction-modification system subunit M (Bacteroides stercoris CC31F)
MATNNTTEQSLTKKVWSLATTLAGQGIGFTDYITQLTYLLFLKMDAENVEMFGEESAIPT
GYQWNDLIALDGLDLVKQYEETLKLLSEQDNLIGTIYTKAQNKIDKPVYLKKVITMIDEE
QWLIMDGDVKGAIYESILEKNGQDKKSGAGQYFTPRPLIQAMVDCINPQMGETVCDPACG
TGGFLLAAYDYMKVQSASKEKRDFLRDKALHGVDNTPLVVTLASMNLYLHGIGTDRSPIV
CEDSLEKEPSTLVDVILANPPFGTRPAGSVDINRPDFYVETKNNQLNFLQHMMLMLKTGG
RAAVVLPDNVLFEAGAGETIRKRLLQDFNLHTILRLPTGIFYAQGVKANVLFFSKGQPTK
EIWFYDYRTDVKHTLATNKLERHHLDDFISCYNNRVETYDAENNPQGRWRKYPIEDILVR
DKTSLDITWIKQGGEADNRSLAELMADIKDKSQTISSAVAELEKLLANINED