Protein Info for HMPREF1181_RS11305 in Bacteroides stercoris CC31F

Annotation: bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 4 to 119 (116 residues), 140.4 bits, E=3e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 122 to 289 (168 residues), 221.4 bits, E=4.1e-70

Best Hits

Swiss-Prot: 96% identical to FOLD_BACFN: Bifunctional protein FolD (folD) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 96% identity to bth:BT_1607)

MetaCyc: 49% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• L-histidine degradation III (6/6 steps found)
• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (7/9 steps found)
• folate transformations I (9/13 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation II (anaerobic) (12/24 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (293 amino acids)

>HMPREF1181_RS11305 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD (Bacteroides stercoris CC31F)
MTLIDGKAISEQVKQEIATEVAEIVARGGKHPHLAAILVGHDGGSETYVAAKVKACEACG
FKSSLIRYETDVTEEELLAKVRELNEDADVDGFIVQLPLPKHISEQKVIETIDYRKDVDG
FHPINVGRMSIGLPCYVSATPNGILELLKRYKIETSGKKCVVLGRSNIVGKPMAALMMQK
AYPGDATVTVCHSRSKDLVKECQEADIIIAAMGQPNFVKAEMVKEGAVVIDVGTTRVPDA
TRKSGFKLTGDVKFDEVAPKCSFITPVPGGVGPMTIVSLMKNTLLAGKKAIYQ