Protein Info for HMPREF1181_RS10970 in Bacteroides stercoris CC31F

Annotation: replicative DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 TIGR00665: replicative DNA helicase" amino acids 6 to 443 (438 residues), 400.4 bits, see alignment E=4.7e-124 PF00772: DnaB" amino acids 6 to 107 (102 residues), 87.1 bits, see alignment E=1.1e-28 PF03796: DnaB_C" amino acids 183 to 443 (261 residues), 281 bits, see alignment E=1.3e-87 PF13481: AAA_25" amino acids 188 to 364 (177 residues), 41.2 bits, see alignment E=2.2e-14

Best Hits

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 66% identity to bth:BT_1516)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (458 amino acids)

>HMPREF1181_RS10970 replicative DNA helicase (Bacteroides stercoris CC31F)
MAEILNPQDAELEEVILGACLIGREAMPMVADRLRPEMFYFDKNALIYAAMQVMYREGRQ
IDIITVKNELVARGKLDAAGGPYELTRISGRVASAVHLEYHVLILREKYLRREMILGFHK
LLALAADETTDIGDTLVDAHNLLDRLEGECGTIEHLRDMDTLMDDTLQQVDARQASGSNG
VTGISTGLADLDRLTSGWQRGDLNVIAARPAVGKTAFALHLARAAATAGHHVAVYSLEMQ
GERLGDRWLIAASPDVNARHLRSGQLTDDEVAQVRTAASELRVLPIHVDDHPVTSMDRVR
SSARMLQSKGKCDLIILDYLQLCDMKSDQKNRNREQEVAQTTRKAKLMAKELNVPVLLLS
QLNRGSDGRPFSRPILSDLRESGAIEQDADMVMLLYRPALAGIRTEPVSKYPSEHLGVVI
VAKHRNGETGDVYFAHNPSMTKLGEYVPPTEWLMRNAK