Protein Info for HMPREF1181_RS10690 in Bacteroides stercoris CC31F

Annotation: alanine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 TIGR00518: alanine dehydrogenase" amino acids 1 to 365 (365 residues), 529.8 bits, see alignment E=1.7e-163 PF05222: AlaDh_PNT_N" amino acids 4 to 137 (134 residues), 168 bits, see alignment E=2.6e-53 PF01262: AlaDh_PNT_C" amino acids 141 to 353 (213 residues), 295 bits, see alignment E=5.8e-92 PF02826: 2-Hacid_dh_C" amino acids 166 to 268 (103 residues), 31.7 bits, see alignment E=1.9e-11 PF01488: Shikimate_DH" amino acids 167 to 269 (103 residues), 24.4 bits, see alignment E=5.6e-09

Best Hits

Swiss-Prot: 58% identical to DHA_HALED: Alanine dehydrogenase (ald) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K00259, alanine dehydrogenase [EC: 1.4.1.1] (inferred from 89% identity to bhl:Bache_1992)

MetaCyc: 58% identical to alanine dehydrogenase subunit (Klebsiella aerogenes)
Alanine dehydrogenase. [EC: 1.4.1.1]

Predicted SEED Role

"Alanine dehydrogenase (EC 1.4.1.1)" in subsystem Pyruvate Alanine Serine Interconversions (EC 1.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (368 amino acids)

>HMPREF1181_RS10690 alanine dehydrogenase (Bacteroides stercoris CC31F)
MIIGVPKEIKNNENRVGMTPAGVAELVKKGHTVYVQATAGANSGFSDDDYIAVGAQILPA
IEDVYAIADMIVKVKEPIAPEYKLIKKGQVVFTYFHFAADKLLTEAMIESGAVCIAYETV
EKDDHSLPLLTPMSEVAGRMATQVGARFLEKPQGGKGKLMGGVPGVRPARVLILGGGVVG
TNAAQMAAGMGAEVMITDVNLTRLRYLSEVLPKNVKTLYSSLHNIKMELPTVDLVIGSVL
IPGDKAPHLITREMLKMMKPGTVLVDVAIDQGGCFETSHPTTHSEPTYVVDGIVHYAVAN
IPGAVPFTSTMALTNATLPYTVALACKGWKQACKDDPALALGLNVVEGKVTYKAVADVFG
MRYEEIEL